Part-Time Laboratory Technician

St. John; New York (NY); Presencial; USD 30.000 - 34.000; A tiempo parcial

Descripción de la vacante

St. John's University in Queens, NY, seeks a Part-Time Laboratory Technician to support NIH-funded molecular biology projects.

The role emphasizes hands-on Western blotting, cell culture, and related experiments, with an emphasis on reproducible data and careful documentation. The successful candidate will work closely with faculty and students, establish standard procedures, and contribute to training of lab members, while complying with safety and biosafety requirements in a biomedical

Formación

• Bachelor's degree or equivalent combination of education and hands-on laboratory experience.
• Demonstrated hands-on experience performing Western blotting independently.
• Demonstrated ability to follow experimental protocols accurately and generate reproducible results.

Responsabilidades

• Independently perform Western blotting experiments and data analysis.
• Maintain detailed experimental records and sample information.
• Perform mammalian cell culture and prepare samples for biochemical analysis.
• Assist with NIH-funded research projects and establish standardized lab procedures.

Conocimientos

Western blotting

Mammalian cell culture

Laboratory techniques

Experimental documentation

Attention to detail

Problem solving

Communication

Safety awareness

Educación

Bachelor's degree or equivalent in Biology or related field

Herramientas

LI-COR Odyssey

Western blotting equipment

Descripción del empleo

Part-Time Laboratory Technician

St. John's University

Salary: $22.00 - $25.00 Hourly

Job Type: Part-Time Staff

Job Number: 202600085

Location: Queens Campus, NY

St. John's, established in 1870, has two New York City campuses;international locations in Rome, Italy; Limerick, Ireland; andParis, France; and study abroad locations around the world. ThePrinceton Review and other top rankings consistently recognize theUniversity's outstanding academics, diverse student body, dynamicinternship, and volunteer opportunities, focus on student life, anddiverse study abroad offerings. St. John's University offers morethan 100 undergraduate and graduate programs in its six collegesand schools, with a growing number of programs offered online. TheUniversity is accredited by the Middle States Commission on HigherEducation and 12 other major academic and professionalassociations.

A dedication to diversity, equity and inclusion is at the heart ofour mission. As a Catholic and Vincentian university, St. John's iscommitted to institutionalizing practices of inclusive excellenceto ensure that we welcome and celebrate the intrinsic worth of allmembers of our community. We will become an even strongeruniversity as we enhance equity at every level of ourinstitution.Our graduates will excel in the competencies and valuesneeded for leadership and service in a rapidly evolvingworld.

Job Summary

This part-time laboratory technician position supports an NIHR16-funded research program in the Department of BiologicalSciences. The primary responsibility of this position is to performWestern blotting and related cell and molecular biology experimentsand to generate high-quality, reproducible experimental data.

The ideal candidate will have substantial hands‑on experience withWestern blotting and should be able to perform the techniqueindependently, including sample preparation, SDS-PAGE, proteintransfer, antibody incubation, imaging, data analysis, andtroubleshooting. Experience with mammalian cell culture and othermolecular and cellular biology techniques is also desirable.

The technician will work closely with faculty and students and mayassist in establishing standardized laboratory procedures andtraining laboratory members in Western blotting and relatedtechniques. This position has no supervisory or leadershipresponsibilities

Job Responsibilities

• Independently perform Western blotting experiments, includingprotein sample preparation, SDS-PAGE, membrane transfer, blocking,primary and secondary antibody incubation, washing, imaging, andquantitative analysis.
• Optimize and troubleshoot Western blotting conditions to obtainreproducible, publication-quality data.
• Maintain detailed experimental records, including sampleinformation, experimental conditions, raw images, and quantitativeresults.
• Perform mammalian cell culture and prepare cell lysates forbiochemical analysis.
• Assist with routine molecular and cellular biology experimentsrelated to the NIH-funded research project.
• Establish and follow standardized Western blotting procedures to improve experimental reproducibility within the laboratory.
• Assist faculty and students with Western blotting techniques andtroubleshooting as needed.
• Prepare laboratory reagents, buffers, and stocks required forWestern blotting and related experiments.
• Maintain and appropriately use laboratory equipment related toprotein analysis and cell culture.
• Follow University laboratory safety, biosafety, and researchcompliance requirements.
• Communicate experimental progress, technical problems, andresults clearly with the Principal Investigator and other researchteam members.

Competencies, Education, Experience

Required Education and Experience:

• Bachelor's degree or an equivalent combination of education andrelevant hands‑on laboratory experience.
• Demonstrated hands‑on experience performing Western blottingindependently.
• Demonstrated ability to follow experimental protocols accuratelyand generate reproducible laboratory results.

Preferred Education and Experience:

• Bachelor's degree in Biology, Biochemistry, Molecular Biology,Chemistry, Biotechnology, or a related scientific field.
• Previous research experience in an academic, biotechnology,pharmaceutical, or biomedical research laboratory.
• Substantial hands‑on experience with Western blotting and proteinanalysis.
• Experience with mammalian cell culture and related molecular orcellular biology techniques.

Required Qualifications and Skills

• Western blotting proficiency: Demonstrated hands‑on experienceperforming Western blotting independently and producing consistent,high-quality results.
• Western blot troubleshooting: Ability to identify and correctcommon problems involving sample preparation, electrophoresis,protein transfer, antibody conditions, background, signalintensity, and imaging.
• Experimental reproducibility: Ability to follow standardizedprocedures carefully and reproduce experimental results acrossindependent experiments.
• Laboratory technique: Strong pipetting, solution preparation,sample handling, and general laboratory skills.
• Experimental documentation: Ability to maintain accurate andorganized laboratory records and appropriately document rawexperimental data.
• Attention to detail: High level of accuracy in samplepreparation, reagent preparation, experimental execution, andrecordkeeping.
• Problem solving: Ability to recognize technical problems andindependently identify reasonable troubleshooting approaches.
• Communication and teamwork: Ability to communicate effectivelyand work collaboratively with faculty, graduate students, andundergraduate researchers.
• Safety awareness: Understanding of and adherence to laboratorysafety and biosafety procedures.

Preferred Qualifications and Skills

• Experience performing Western blotting in a biomedical researchlaboratory, particularly where Western blot data were used forresearch presentations, manuscripts, or publications.
• Experience with mammalian cell culture.
• Experience preparing protein lysates and performing proteinquantification assays.
• Experience with fluorescence-based Western blot imaging andquantitative analysis.
• Experience with LI-COR Odyssey or similar fluorescence imagingsystems.
• Familiarity with Image Studio or other software used for Westernblot quantification.
• Experience with molecular and cellular biology techniques such asfluorescence microscopy, transfection, or cell-based assays.
• Experience training or assisting other laboratory personnel withWestern blotting techniques.

Supervisory Responsibility

Not applicable. The position may provide technical guidance andhands‑on assistance to students performing Western blotting andrelated laboratory procedures but does not have formal supervisoryresponsibility.

Additional Eligibility Qualifications

• Ability to complete mandatory University laboratory safety andcompliance training.
• Ability to work independently after appropriate orientation tolaboratory-specific procedures.
• Commitment to accurate recordkeeping, research integrity, andreproducible experimental practices.
• Willingness to participate in a standardized technical skillsassessment as part of the selection process, if necessary.

Other Duties

Please note that this job description is not designed to cover orcontain a comprehensive listing of all activities, duties, orresponsibilities required of the employee. Duties andresponsibilities may change as needed to meet the requirements ofthe NIH-funded research program and St. John's University.

Supplemental Information

Work Environment

This position operates in a biomedical research laboratory withinthe Department of Biological Sciences. The technician willroutinely use laboratory equipment including micropipettes,centrifuges, electrophoresis and protein-transfer systems,fluorescence imaging equipment, biosafety cabinets, incubators,microscopes, balances, and computers.

The work involves handling mammalian cell cultures, biologicalsamples, antibodies, chemicals, and common laboratory reagents inaccordance with institutional safety and biosafetyrequirements.

Physical Demands

While performing the duties of this job, the employee is regularlyrequired to sit, stand, move within the laboratory, and use handsfor precise laboratory procedures such as pipetting and samplehandling.

The employee may occasionally be required to lift or movelaboratory supplies or equipment weighing up to 20 lbs. Closevision and the ability to distinguish and evaluate experimentalsamples and images are required.

Environmental Conditions

The work environment may involve routine exposure to:

• Common laboratory chemicals, reagents, odors, or vapors.
• Biological samples and mammalian cell cultures.
• Laboratory equipment including centrifuges, electrophoresissystems, transfer equipment, imaging systems, incubators, andbiosafety cabinets.
• Sharps and other routine laboratory hazards.

Appropriate personal protective equipment, safety training, andlaboratory-specific training will be provided. The employee mustcomply with St. John's University and applicable federal laboratorysafety and biosafety requirements.

Position Type/Expected Hours of Work

This is a part-time position contingent upon the availability ofNIH R16 grant funding. The anticipated workload is approximately 20hours per week, with scheduling arranged during standard businesshours Monday through Friday.

Some flexibility in scheduling may be necessary because certainexperimental procedures, including Western blotting and cellculture, may require work to be performed at specific times.

Travel

Not applicable.

*Important Note- St. John's is NOT an E-VerifyEmployer

St. John's offers a competitive compensation program which iscommensurate with your qualifications, experience, and contingentupon the departmental budget. St. John's University considersfactors such as (but not limited to) scope and responsibilities ofthe position, candidate's work experience, education/training, keyskills, internal peer equity, as well as market and organizationalconsiderations when extending an offer.

We also offer an extremely comprehensive benefits program to meetthe diverse needs of our workforce. Along with exceptional benefitssuch as medical, dental, life insurance, long term disabilityinsurance, tuition remission, generous 403(b) employercontribution, employee assistance program, and liberal paid timeoff policies, faculty and staff can also enjoy St. John'sperforming arts, libraries, bookstores, dining facilities, campusrecreation and sporting events

Any offer of employment is subject to receipt by St. John'sUniversity of satisfactory references, verification of employmentand education.

St. John's University is an Equal Opportunity Employer. Allqualified applicants will receive consideration without regard torace, color, religion, sex, sexual orientation, gender identity,national or ethnic origin, age, status as an individual with adisability, protected veteran status, or any other characteristicprotected by law.