Part-Time Laboratory Technician

St.-John; New York (NY); Presencial; USD 28 000 - 44 000; Tempo parcial

Resumo da oferta

St. John’s University in New York is seeking a part-time Laboratory Technician to support an NIH R16-funded research program in the Department of Biological Sciences. The role focuses on Western blotting, cell culture, and generating high-quality data.

The candidate will work closely with faculty and students and help establish standardized procedures. Ideal applicants will have substantial hands-on Western blot experience and be capable of independent work, data analysis, and careful

Qualificações

• Bachelor’s degree or equivalent with hands-on lab experience.
• Demonstrated hands-on Western blotting experience.
• Ability to follow fixed protocols and generate reproducible results.

Responsabilidades

• Independently perform Western blotting experiments and related steps.
• Optimize and troubleshoot blotting conditions for reproducible data.
• Maintain detailed experimental records and quantitative results.
• Perform mammalian cell culture and prepare lysates for analysis.
• Assist with routine molecular biology experiments related to NIH-funded project.
• Establish and follow standardized blotting procedures to improve reproducibility.
• Assist faculty and students with blotting techniques and troubleshooting.

Conhecimentos

Western blotting

Mammalian cell culture

Data analysis

Troubleshooting

Laboratory techniques

Documentation

Safety compliance

Teamwork

Formação académica

Bachelor’s degree or equivalent

Bachelor’s degree in Biology or related field

Ferramentas

LI-COR Odyssey

Imaging systems

Microscopy

Descrição da oferta de emprego

This part-time laboratory technician position supports an NIH R16-funded research program in the Department of Biological Sciences. The primary responsibility of this position is to perform Western blotting and related cell and molecular biology experiments and to generate high-quality, reproducible experimental data.

The ideal candidate will have substantial hands‑on experience with Western blotting and should be able to perform the technique independently, including sample preparation, SDS‑PAGE, protein transfer, antibody incubation, imaging, data analysis, and troubleshooting. Experience with mammalian cell culture and other molecular and cellular biology techniques is also desirable.

The technician will work closely with faculty and students and may assist in establishing standardized laboratory procedures and training laboratory members in Western blotting and related techniques. This position has no supervisory or leadership responsibilities

Responsibilities

• Independently perform Western blotting experiments, including protein sample preparation, SDS‑PAGE, membrane transfer, blocking, primary and secondary antibody incubation, washing, imaging, and quantitative analysis.
• Optimize and troubleshoot Western blotting conditions to obtain reproducible, publication-quality data.
• Maintain detailed experimental records, including sample information, experimental conditions, raw images, and quantitative results.
• Perform mammalian cell culture and prepare cell lysates for biochemical analysis.
• Assist with routine molecular and cellular biology experiments related to the NIH-funded research project.
• Establish and follow standardized Western blotting procedures to improve experimental reproducibility within the laboratory.
• Assist faculty and students with Western blotting techniques and troubleshooting as needed.
• Prepare laboratory reagents, buffers, and stocks required for Western blotting and related experiments.
• Maintain and appropriately use laboratory equipment related to protein analysis and cell culture.
• Follow University laboratory safety, biosafety, and research compliance requirements.
• Communicate experimental progress, technical problems, and results clearly with the Principal Investigator and other research team members.

Required Education and Experience:

• Bachelor’s degree or an equivalent combination of education and relevant hands‑on laboratory experience.
• Demonstrated hands‑on experience performing Western blotting independently.
• Demonstrated ability to follow experimental protocols accurately and generate reproducible laboratory results.

Preferred Education and Experience:

• Bachelor’s degree in Biology, Biochemistry, Molecular Biology, Chemistry, Biotechnology, or a related scientific field.
• Previous research experience in an academic, biotechnology, pharmaceutical, or biomedical research laboratory.
• Substantial hands‑on experience with Western blotting and protein analysis.
• Experience with mammalian cell culture and related molecular or cellular biology techniques.

Required Qualifications and Skills

• Western blotting proficiency: Demonstrated hands‑on experience performing Western blotting independently and producing consistent, high-quality results.
• Western blot troubleshooting: Ability to identify and correct common problems involving sample preparation, electrophoresis, protein transfer, antibody conditions, background, signal intensity, and imaging.
• Experimental reproducibility: Ability to follow standardized procedures carefully and reproduce experimental results across independent experiments.
• Laboratory technique: Strong pipetting, solution preparation, sample handling, and general laboratory skills.
• Experimental documentation: Ability to maintain accurate and organized laboratory records and appropriately document raw experimental data.
• Attention to detail: High level of accuracy in sample preparation, reagent preparation, experimental execution, and recordkeeping.
• Problem solving: Ability to recognize technical problems and independently identify reasonable troubleshooting approaches.
• Communication and teamwork: Ability to communicate effectively and work collaboratively with faculty, graduate students, and undergraduate researchers.
• Safety awareness: Understanding of and adherence to laboratory safety and biosafety procedures.

Preferred Qualifications and Skills

• Experience performing Western blotting in a biomedical research laboratory, particularly where Western blot data were used for research presentations, manuscripts, or publications.
• Experience with mammalian cell culture.
• Experience preparing protein lysates and performing protein quantification assays.
• Experience with fluorescence‑based Western blot imaging and quantitative analysis.
• Experience with LI‑COR Odyssey or similar fluorescence imaging systems.
• Familiarity with Image Studio or other software used for Western blot quantification.
• Experience with molecular and cellular biology techniques such as fluorescence microscopy, transfection, or cell‑based assays.
• Experience training or assisting other laboratory personnel with Western blotting techniques.

Supervisory Responsibility

Not applicable. The position may provide technical guidance and hands‑on assistance to students performing Western blotting and related laboratory procedures but does not have formal supervisory responsibility.

Additional Eligibility Qualifications

• Ability to complete mandatory University laboratory safety and compliance training.
• Ability to work independently after appropriate orientation to laboratory‑specific procedures.
• Commitment to accurate recordkeeping, research integrity, and reproducible experimental practices.
• Willingness to participate in a standardized technical skills assessment as part of the selection process, if necessary.

Other Duties

Please note that this job description is not designed to cover or contain a comprehensive listing of all activities, duties, or responsibilities required of the employee. Duties and responsibilities may change as needed to meet the requirements of the NIH‑funded research program and St. John’s University.

Work Environment

This position operates in a biomedical research laboratory within the Department of Biological Sciences. The technician will routinely use laboratory equipment including micropipettes, centrifuges, electrophoresis and protein‑transfer systems, fluorescence imaging equipment, biosafety cabinets, incubators, microscopes, balances, and computers.

The work involves handling mammalian cell cultures, biological samples, antibodies, chemicals, and common laboratory reagents in accordance with institutional safety and biosafety requirements.

Physical Demands

While performing the duties of this job, the employee is regularly required to sit, stand, move within the laboratory, and use hands for precise laboratory procedures such as pipetting and sample handling.

The employee may occasionally be required to lift or move laboratory supplies or equipment weighing up to 20 lbs. Close vision and the ability to distinguish and evaluate experimental samples and images are required.

Environmental Conditions

• Common laboratory chemicals, reagents, odors, or vapors.
• Biological samples and mammalian cell cultures.
• Laboratory equipment including centrifuges, electrophoresis systems, transfer equipment, imaging systems, incubators, and biosafety cabinets.
• Sharps and other routine laboratory hazards.

Appropriate personal protective equipment, safety training, and laboratory‑specific training will be provided. The employee must comply with St. John’s University and applicable federal laboratory safety and biosafety requirements.

Position Type/Expected Hours of Work

This is a part‑time position contingent upon the availability of NIH R16 grant funding. The anticipated workload is approximately 20 hours per week, with scheduling arranged during standard business hours Monday through Friday.

Some flexibility in scheduling may be necessary because certain experimental procedures, including Western blotting and cell culture, may require work to be performed at specific times.

Travel

Not applicable.