Lentiviral T-Cell Scientist for Immunotherapy

Tulynode Biosciences Inc; San Diego (CA); On-site; USD 90,000 - 130,000; Full time

Job summary

Tulynode Biosciences Inc. is seeking a motivated Scientist/Senior Research Associate to join the Discovery and Cell Therapy team.

The role focuses on engineering tumor-infiltrating lymphocytes (TILs) using lentiviral vectors and supporting molecular cloning, transduction workflows, and functional assays. Ideal candidates have 1–3+ years of post-grad experience, strong molecular biology skills, and familiarity with FlowJo data analysis.

Qualifications

• Master or B.S. in molecular biology, immunology, biotechnology, cell biology, or related discipline.
• 1–3+ years of post-grad lab experience, biotech setting preferred.
• Proficiency in aseptic mammalian cell culture, ideally with T cells or TILs.
• Experience with transfection methods (e.g., HEK293T packaging) and lentiviral transduction workflows.
• Foundational molecular biology skills (PCR, cloning, Gibson Assembly).
• Experience with multi-color flow cytometry panels and data analysis using FlowJo.
• Exposure to T-cell therapy expansion, bioreactors, or scale-up workflows.
• Knowledge of T-cell exhaustion markers and tumor microenvironment mechanisms.
• Strong organizational and communication skills for a fast-paced team.

Responsibilities

• Lentiviral vector production, concentration, and titering; assist in transfer and packaging plasmids.
• Isolate, expand, and modify primary T cells and TILs using aseptic culture techniques; optimize transduction workflows.
• Perform routine molecular biology tasks: plasmid cloning, DNA/RNA isolation, qPCR/RT-qPCR, Sanger sequencing.
• Conduct phenotypic and functional assays, including flow cytometry and cytotoxicity or cytokine profiling.
• Maintain ELN records; analyze datasets with FlowJo/GraphPad; present findings to cross-functional teams.

Skills

Aseptic mammalian cell culture

Lentiviral transduction

Molecular biology techniques

Flow cytometry (FlowJo)

T-cell therapy basics

Data analysis (FlowJo/GraphPad)

Education

Master's or Bachelor's in Molecular Biology/Immunology/Biotechnology/Cell Biology

Bachelor's in Molecular Biology/Immunology/Biotechnology/Cell Biology

Tools

FlowJo

GraphPad Prism

FACS hardware

Job description

Tulynode Biosciences Inc. is seeking a motivated Scientist/Senior Research Associate to join the Discovery and Cell Therapy team.

The role focuses on engineering tumor-infiltrating lymphocytes (TILs) using lentiviral vectors and supporting molecular cloning, transduction workflows, and functional assays. Ideal candidates have 1–3+ years of post-grad experience, strong molecular biology skills, and familiarity with FlowJo data analysis.