Junior Specialist – Erlebacher lab

University of California San Francisco; San Francisco (CA); On-site; USD 57,000 - 61,000; Full time

Job summary

University of California San Francisco seeks a Junior Specialist for the Erlebacher lab at the Parnassus Campus to contribute to immunology and epigenetics research on pregnancy. The role involves independent project work and a range of techniques in a basic/translational science setting.

The position includes immunohistochemistry, RNA extraction, PCR/qPCR, cell culture, mouse genetics, and thoughtful lab management, performed under supervision with strong organizational and communication skills.

Qualifications

• Bachelor's degree or four years of research experience required.
• Familiarity with basic molecular biology, biochemical and microbiological techniques.
• Computer skills including Excel.

Responsibilities

• Immunohistochemical and immunofluorescence staining of tissue sections.
• RNA extraction, PCR, qPCR, RT-PCR, and Western blots; primer design.
• Cell culture, transfections, lentivirus preparation.
• Mouse colony maintenance and genotyping; record keeping.
• Mouse pregnancy experiments and labor timing studies.
• Isolation of cells from tissues; flow cytometry and single-cell RNA-Seq.
• Lab management: reagent orders, supplies, EH&S and IACUC liaison.

Skills

Molecular biology techniques

Cell culture

Flow cytometry

PCR/qPCR/RT-PCR

Education

Bachelor Degree in science or related majors

Tools

Excel

Job description

Junior Specialist – Erlebacher lab

The Junior Specialist will work in a basic/translational science laboratory on the UCSF Parnassus Campus that focuses on immunological and epigenetic features of pregnancy including why the fetus is not rejected by the maternal immune system, how labor induction is timed and controlled, and causes of pregnancy complications (adrianerlebacherlab.org). The work builds upon the lab’s recent publications in Cell, Immunity and Nature. Under the supervision of the PI and lab members, s/he will work independently on multiple projects and perform a variety of techniques, including:

• Immunohistochemical and immunofluorescence staining of tissue sections, including photomicroscopy
• Molecular biology techniques including RNA extraction, PCR, qPCR, RT-PCR, western blots, and PCR primer design using online programs
• Cell culture, including cell transfections and treatments, and lentivirus preparation
• Mouse colony maintenance, including tailing mice for genotyping, weaning of mouse litters, preparation of tail DNA and PCR analysis, and record keeping
• Mouse pregnancy experiment, including drug administration and labor induction timing experiments
• Isolation of cells from mouse tissues, and their analysis by a variety of techniques, including flow cytometry, single cell RNA-Seq, and CUT&RUN
• Lab managerial duties, including reagent and supply orders, maintenance of laboratory supplies, and interfacing with EH&S and IACUC

The applicant must be able to perform detail-oriented tasks, manage multiple tasks at any one time, prioritize and perform experiments with minimal supervision, and have excellent organizational and communication skills.

Appointees in the Specialist series will be expected to engage in specialized research, professional activities and do not have teaching responsibilities. Specialists are expected to use their professional expertise to make scientific and scholarly contributions, and may participate in University and Public Service.

Required Experience, Skills

• Bachelor Degree (or equivalent degree) in science or related majors or at least four years of research experience.
• familiarity with basic molecular biological, biochemical, and microbiological techniques.
• computer skills including the use of Excel.

A bachelor’s degree is preferred. Candidates must meet the qualifications by the time of appointment. Application materials must state qualifications or if pending upon submission.

Screening of applicants will begin immediately and will continue as needed throughout the recruitment period. Salary will be commensurate with the applicants experience and training.

A reasonable estimate for this position is $57,000-$60,700.

Bachelor Degree (or equivalent degree) in science or related majors or at least four years of research experience.

Application materials must state qualifications or if pending upon submission.