Immunology Research Scientist (CRISPR & Flow Cytometry)

The University of Iowa; Iowa City (IA); Presencial; USD 51.000 - 62.000; Jornada completa

Descripción de la vacante

The University of Iowa's Department of Pathology seeks an Assistant Research Scientist to investigate how A-FABP and E-FABP regulate cell metabolism and intracellular signaling in disease models such as obesity, cancer, and psoriasis. You will design and execute research projects and generate high-quality data, while mentoring lab personnel and coordinating protocols.

This on-campus, full-time position involves training students and postdocs, maintaining mouse colonies, performing flow

Formación

• Ph.D. in Immunology required.
• At least 1 year of postdoctoral experience in Immunology or cancer-related field.
• Demonstrated ability to independently design, optimize and perform experiments.
• Evidence of publications in peer-reviewed journals.
• Experience presenting at professional meetings.
• Excellent communication and teamwork skills.

Responsabilidades

• Cell culture for various cell lines and CRISPR-derived lines.
• Prepare single-cell suspensions from tissues.
• Perform real-time PCR, Western blotting, and RNAi.
• Generate, isolate and purify antibodies.
• Perform ELISA from serum and supernatants.
• Immunofluorescence staining of cells and tissues.
• Maintain mouse colonies and conduct genotyping with technician support.
• Prepare and analyze samples using multi-color flow cytometry.
• Troubleshoot procedures and update protocols as needed.

Conocimientos

Cell culture

Real-time PCR

Western blotting

RNA interference (RNAi)

Immunofluorescence

Genotyping

Team collaboration

Educación

Ph.D. in Immunology

Postdoctoral experience in Immunology or cancer

Herramientas

CRISPR gene editing

Flow cytometry

Mouse handling & husbandry

ELISA

Descripción del empleo

The University of Iowa's Department of Pathology seeks an Assistant Research Scientist to investigate how A-FABP and E-FABP regulate cell metabolism and intracellular signaling in disease models such as obesity, cancer, and psoriasis. You will design and execute research projects and generate high-quality data, while mentoring lab personnel and coordinating protocols.

This on-campus, full-time position involves training students and postdocs, maintaining mouse colonies, performing flow