Asst Research Scientist/Engin - Li Lab

The University of Iowa; Iowa City (IA); On-site; USD 51,000 - 62,000; Full time

Job summary

The University of Iowa's Department of Pathology seeks an Assistant Research Scientist to investigate how A-FABP and E-FABP regulate cell metabolism and intracellular signaling in disease models such as obesity, cancer, and psoriasis. You will design and execute research projects and generate high-quality data, while mentoring lab personnel and coordinating protocols.

This on-campus, full-time position involves training students and postdocs, maintaining mouse colonies, performing flow

Qualifications

• Ph.D. in Immunology required.
• At least 1 year of postdoctoral experience in Immunology or cancer-related field.
• Demonstrated ability to independently design, optimize and perform experiments.
• Evidence of publications in peer-reviewed journals.
• Experience presenting at professional meetings.
• Excellent communication and teamwork skills.

Responsibilities

• Cell culture for various cell lines and CRISPR-derived lines.
• Prepare single-cell suspensions from tissues.
• Perform real-time PCR, Western blotting, and RNAi.
• Generate, isolate and purify antibodies.
• Perform ELISA from serum and supernatants.
• Immunofluorescence staining of cells and tissues.
• Maintain mouse colonies and conduct genotyping with technician support.
• Prepare and analyze samples using multi-color flow cytometry.
• Troubleshoot procedures and update protocols as needed.

Skills

Cell culture

Real-time PCR

Western blotting

RNA interference (RNAi)

Immunofluorescence

Genotyping

Team collaboration

Education

Ph.D. in Immunology

Postdoctoral experience in Immunology or cancer

Tools

CRISPR gene editing

Flow cytometry

Mouse handling & husbandry

ELISA

Job description

The Department of Pathology is seeking an Assistant Research Scientist to investigate the mechanisms of how A-FABP and E-FABP regulate cell metabolism and intracellular signaling pathways in cells from various types of disease models like obesity, cancer and psoriasis. Tasks include the design and performance assigned research project and obtain high-quality data in each individual study. Tasks also include management of laboratory protocols, training and collaboration of laboratory personnel, including students, post-doctoral fellows and technicians. This position is 100% time.

The Department of Pathology is part of UI Health Care, a comprehensive health sciences center that serves Iowa and the region, as well as patients referred from the United States and overseas. The Clinical Laboratories are well recognized for providing outstanding and comprehensive care to the patients we serve. The breadth and depth of services provided are amongst the best in the country. The educational programs that the department participates in includes medical students, clinical laboratory scientists as well as biomedical scientists. To learn more: https://pathology.medicine.uiowa.edu/about-us

For a full job description, please send an e-mail to the contact listed below.

Duties to include:

• Cell culture for cell lines, primary immune cells like BMDC, BMDM, establishing cell lines of stable gene expression or knockout using CRISPR technique;
• Preparation of single cell suspension from tissues.
• Highly skilled on real-time PCR, western blotting, RNAi;
• Perform antibody generation, isolation and purification;
• Perform ELISA from serum and supernatant;
• Perform Immunofluorescence staining using cells and tissues;
• Maintenance of mouse colonies and genotyping with assistant of technician;
• Prepare, perform and analyze samples using multi-color flow cytometry;
• Troubleshoots with issues for experimental procedures and updates as needed.

Required Qualifications:

• Ph.D. in Immunology
• At least 1 year of postdoctoral experience in Immunology or cancer-related field.
• Demonstration of strong ability to independently design, optimize and perform experiments, establishing experimental protocols to solve scientific questions.
• Evidence with a record of publications in peer-reviewed journals.
• Experience of establishing a national presence with talks, abstract presentations and/or participation at professional meetings.
• Excellent communication skills and integrating positive effort to teamwork.

Desirable Qualifications:

• At least 3 years of postdoctoral experience in Immunology or cancer-related field.
• Prior experience with developing mouse models on high fat diet/tumor implantation, and compliance with IACUC regulations is highly desired.
• Highly skilled in multi-color Flow cytometry to identify immune cells (T cell, macrophages) is highly desired.
• Standard molecular biological and immunological techniques is highly desired.

Job openings are posted for a minimum of 7 calendar days and may be removed from posting and filled any time after the original posting period has ended.

Successful candidates will be subject to a criminal background check and credential/education verification. Up to 5 professional references will be requested at a later step in the recruitment process.

Job Details

• Classification Title: Asst Research Scientist/Engin
• Appointment Type: Professional and Scientific
• Schedule: Full-time
• Work Modality Options: On Campus

Compensation

• Starting Salary Minimum: $56,697
• Starting Salary Maximum: Commensurate

Contact Information

• Contact Email: [e-mail]

Equal opportunity employer

The University of Iowa is an equal opportunity employer. All qualified applicants are encouraged to apply and will receive consideration for employment free from discrimination on the basis of race, creed, color, religion, national origin, age, sex, pregnancy (including childbirth and related conditions), disability, genetic information, status as a U.S. veteran, service in the U.S. military, sexual orientation, or associational preferences.

Persons with disabilities who need assistance or accommodations with the application or interview process may contact University Human Resources/Faculty and Staff Disability Services, [phone] or [e-mail] . For jobs in UI Health care, please contact UI Health care Leave & Disability Administration at 319-356-7543 .