E11 Bio - Scientist/Senior Scientist, Molecular Engineering & Sample Optimization

Ring Inc; Alameda (CA); On-site; USD 100,000 - 240,000; Full time

Job summary

E11 Bio in Alameda, California is seeking a Scientist to help advance brain mapping by developing and deploying assays to assess fixation quality and tissue preservation. You will establish quantitative metrics for protein retention, antigenicity, and labeling across tissue prep conditions.

You will collaborate with in vivo technicians to design experiments, evaluate results, and optimize SOPs, while driving data-analysis pipelines for tissue quality indicators and epitope-binder readouts in

Responsibilities

• Develop and deploy an assay to systematically evaluate and improve the fixation quality and preservation of brain tissue
• Establish and integrate quantitative metrics of molecular readout e.g. protein retention, antigenicity, and labeling quality across tissue preparation conditions
• Work closely with in vivo research technicians to design experiments, evaluate results, and optimize SOPs
• Drive the development of data-analysis pipelines to routinely generate key performance indicators of sample tissue quality
• Generate an in-house pipeline for binder design/discovery, validation, and optimization of epitope-binder pairs in hydrogels to improve the readout of epitope barcode bits and relevant native synaptic proteins
• Develop novel highly multiplexed protein readout strategies for protein barcodes and molecular annotation of synapses and cell types

Job description

E11 Bio is on a mission to advance connectomics towards whole brain scale for humans and other mammals. We are a collaborative and interdisciplinary team of scientists and engineers engaged in a neuroscience moonshot project to develop a radical technology platform for scalable mammalian brain mapping. E11 Bio is a non-profit Convergent Research focused research organization located in Alameda, California, pursuing blue-skies neuroscience research yet operating like a nimble, tight-knit start-up. Read our technology roadmap.

Responsibilities

• Develop and deploy an assay to systematically evaluate and improve the fixation quality and preservation of brain tissue

• Establish and integrate quantitative metrics of molecular readout e.g. protein retention, antigenicity, and labeling quality across tissue preparation conditions

• Work closely with in vivo research technicians to design experiments, evaluate results, and optimize SOPs

• Drive the development of data-analysis pipelines to routinely generate key performance indicators of sample tissue quality

• Generate an in-house pipeline for binder design/discovery, validation, and optimization of epitope-binder pairs in hydrogels to improve the readout of epitope barcode bits and relevant native synaptic proteins

• Develop novel highly multiplexed protein readout strategies for protein barcodes and molecular annotation of synapses and cell types

Outcomes

In the first 6 Months

• Defined key indicators for perfusion quality and deploy an assay to routinely capture these.

• Incorporated protein retention, antigenicity, and specificity as per-stage metrics across the sample generation pipeline.

• Deployed early perfusion or fixed tissue readout metrics that predict the final expanded tissue quality, including both overall preservation and specific markers of cellular health

• Recorded a measurable > 20%improvement in expanded ultrastructural image contrast compared to current baseline perfusion & gelation protocol

• First new epitope-binder designs in production

• Developed assay for binder compatibility in expanded tissue, and validated this assay against existing pairs

• Identified and validated a cocktail of binders for identifying most synapses via e.g. synaptic vesicle binding

In 12 Months:

• Evaluated the first set of designed epitope-binder pairs and synaptic protein-binder pairs and qualified in expanded tissue, 5+ binders exceeding commercial SOTA performance.

• With collaborators, improved the multiplexed readout strategy to allow for routine incorporation into the imaging pipeline and a demonstrated increase in key barcode metrics (e.g. imaging speed or entropy increases)

• Identified and validated multiple cocktails of binders for stratifying excitatory and inhibitory synapses

$100,000 - $191,000 a year

Scientist base salary range: $100K - 165K

Senior Scientist base salary range: $127K - 191K

This position is eligible for a team performance based bonus up to 20% of base salary and a 401k match up to 6% of base salary.

Total potential compensation range: $126K - 240K

E11 Bio, LLC is an Equal Employment Opportunity employer that proudly pursues and hires a diverse workforce. We do not make hiring or employment decisions on the basis of race, color, religion or religious belief, ethnic or national origin, nationality, sex, gender, gender-identity, sexual orientation, disability, age, military or veteran status, or any other basis protected by applicable local, state, or federal laws or prohibited by Company policy. We strive for a healthy and safe workplace and strictly prohibit harassment of any kind.