CRISPR Genome-Editing Postdoctoral Fellow

Children's Hospital of Philadelphia; Philadelphia (Philadelphia County); On-site; USD 21,000 - 110,000; Full time

Job summary

Children’s Hospital of Philadelphia (CHOP) is seeking a postdoctoral fellow in the Pavani Lab to advance genome editing and red cell engineering for translational research. The role focuses on CRISPR/Cas9 and related methods in mammalian cells, with goals to develop novel therapies and improve blood transfusions.

The position is full-time, based in Philadelphia, PA, with mentoring, seminars, and opportunities to publish and contribute to grant proposals.

Qualifications

• PhD in Molecular Biology, Genetics, Cell Biology, Bioengineering, or a related field (or equivalent by start date).
• Hands-on experience with genome editing techniques (CRISPR/Cas or similar).
• Experience with mammalian cell culture and experimental manipulation.
• Practical experience with flow cytometry (sample prep, acquisition, and analysis).

Responsibilities

• Design, optimize, and execute genome-editing experiments in mammalian cells.
• Develop and maintain cell cultures, including iPSCs and immortalized lines.
• Perform genome engineering workflows: vector design and cloning, transfection/electroporation, editing efficiency and genotyping.
• Characterize edited cells using flow cytometry, immunophenotyping, and functional assays.
• Prepare next-generation sequencing (NGS) assays for on-/off-target analysis and deep sequencing of edited loci; analyze sequencing data.
• Troubleshoot experiments, optimize protocols, and maintain rigorous documentation and lab records.
• Present data at internal meetings and conferences; contribute to manuscript and grant preparation.
• Supervise junior lab members, develop new methods and protocols for research, and assist with other projects.

Skills

Genome editing techniques

Mammalian cell culture

Flow cytometry

Respirator use

Education

PhD in Molecular Biology/ Genetics/ Cell Biology/ Bioengineering

Job description

Children’s Hospital of Philadelphia (CHOP) is seeking a postdoctoral fellow in the Pavani Lab to advance genome editing and red cell engineering for translational research. The role focuses on CRISPR/Cas9 and related methods in mammalian cells, with goals to develop novel therapies and improve blood transfusions.

The position is full-time, based in Philadelphia, PA, with mentoring, seminars, and opportunities to publish and contribute to grant proposals.