Biotransformation Scientist

Seventh-Wave-Laboratories-LLC; St. Louis (MO); On-site; USD 110,000 - 160,000; Full time

Benefits offered by this job

Health and dental coverage

Disability insurance

Paid time off

Paid parental leave

401K with company match

Onsite work

Job summary

Inotiv in St. Louis, MO seeks a Biotransformation Scientist to identify enzymatic pathways metabolizing drug candidates using LC-MS/MS and HRMS, with preclinical in vitro and in vivo models including human tissues and subcellular fractions.

You will design studies to establish in vitro clearance mechanisms and in vitro-to-in vivo correlations, collaborate with Pharmacology and Toxicology leadership to develop NAMs, and contribute to predicting human half-life in a dynamic CRO environment.

Qualifications

• PhD in a related field with 2–5 years industrial biotransformation experience.
• Experience with in vitro and in vivo metabolism studies.
• Strong knowledge of LC-MS/MS and HRMS techniques.
• Ability to design mechanistic ADME studies and data interpretation.

Responsibilities

• Design and execute studies to identify enzymatic pathways metabolizing therapeutics.
• Elucidate primary drug clearance mechanisms in vitro and establish in vitro-to-in vivo correlations.
• Collaborate with in vivo pharmacology and toxicology teams on ADME studies.
• Develop NAMs to address mechanistic inquiries and platform screening needs.
• Maintain stringent safety and data integrity in the lab.

Skills

LC-MS/MS

HRMS

Biotransformation

In vitro methods

In vivo pharmacology

Data analysis

Education

PhD in Organic Chemistry

PhD in Biochemistry

Analytical Chemistry MSc/PhD

Postdoctoral experience

Tools

LC-MS/MS instrumentation

HRMS instrumentation

Mass spectrometry data processing

Job description

The Biotransformation Scientist will assist with the identification of enzymatic pathways that metabolize drug candidates in preclinical in vitro and in vivo models, including human tissue and cellular models (e.g., P450, non P450/AO/FMO, conjugative/UGT). Working primarily with preclinical species and human tissues/organ subcellular fractions, the individual will utilize contemporary mass spectrometry techniques (LC-MS/MS, HRMS) to identify and elucidate the structures of drug metabolites (small molecules, peptides, oligonucleotides). The individual will contribute to the development of in vitro-to-in vivo relationships related to drug clearance & half-life predictions. The individual will collaborate with Pharmacology and Toxicology laboratory leadership to establish Novel Approach Methodologies (NAMs) to address mechanistic inquiries and/or platform screening processes for same. Join us in embracing research and science to impact the health and well-being of people all over the world.

Essential Duties and Responsibilities

Design and execute laboratory studies aimed at identifying the enzymatic pathways that metabolize small molecule, peptide, and oligonucleotide therapeutics employing a contemporary approach methodology to establish the primary enzyme(s) contributing to the in vitro metabolism of a drug candidate(s). Design and execute laboratory studies to elucidate primary drug clearance mechanisms in vitro, employing contemporary approach methodology to establish in vitro to in vivo correlations for anticipated drug clearance towards the prediction of human half life. Design and collaborate with in vivo pharmacology & toxicology team members to execute ADME studies (non radiolabeled); elucidating the disposition of drug candidate molecules as it pertains to absorption, distribution, metabolism, and excretion; supplementing with in vitro protein binding, and blood distribution For oligonucleotide and peptide molecules, design and execute in vitro studies aimed at elucidating the exonuclease/endonuclease and peptidase(s) metabolism of drug candidates, respectively. Collaborate with the Toxicology Department to establish cellular models of drug metabolism, induction, and predictive toxicologic mechanisms.

Skills and Abilities

Experience conducting in vitro and ex vivo biotransformation assays utilizing LC–MS/MS & HRMS techniques for quantitation and structure elucidation. Experience, skills, and abilities must include knowledge of contemporary bioanalytical techniques, including wet chemistry separations, liquid chromatography, and mass spectrometric analysis, instrument operation, and data processing. Knowledge of enzyme kinetics desirable. Ability to incorporate software in the maintenance of databases to collate and catalog records and inventory. Independence in the laboratory and data work-up phases of research. A working knowledge and awareness of general laboratory procedures. Good written and oral communication skills. Ability to adhere to all safety regulations and procedures.

Education and Experience

PhD in Organic Chemistry, Biochemistry, Analytical Chemistry or related discipline with 2-5 years direct industrial biotransformation experience following Post Doctoral training M.Sc. in a discipline previously described with 5-7 years direct industrial biotransformation experience Working command of in vitro kinetic analysis and utilization of contemporary mathematical models to effectively analyze, interpret and/or model drug clearance, predicted half life, and drug-drug interactions Ability to perform mass spectrometry data collection, analysis and interpretation experience via LC-MS/MS Candidates possessing a command of Pharmacokinetic modeling, analysis, interpretation and simulation desirable.

Physical Activity and Working Conditions

Work is normally performed in a typical interior office work or laboratory environment Work environment involves exposure to potentially dangerous materials and situations that require following extensive safety precautions and may include use of protective equipment The noise level is usually moderate This position will frequently be involved with radiant/electrical energy, solvents, grease, oil, irritants, acids, bases, and other hazardous chemicals, electro-mechanical hazards, flammable materials and bio-hazards. Due to exposure to hazardous biological material (which may include HIV positive specimens), immunization to Hepatitis B and/or other diseases may be a requirement.

Benefits

Salary will be commensurate with experience and responsibilities.

Benefits include health and dental coverage, short- and long-term disability, paid time off, paid parental leave, 401K participation with company match, and more!

#LI-JM1 #LI-Onsite

Salary and Benefits

Salary will be commensurate with experience and responsibilities.

Benefits include health and dental coverage, short- and long-term disability, paid time off, paid parental leave, 401K, and more!

Equal Opportunity Employer

Inotiv is an Equal Opportunity Employer. It is our policy to provide a fair and equal employment opportunity to all persons, regardless of age, race, color, religion, gender, gender identity, sexual orientation, physical or mental disability, national origin, veteran status, or any other basis prohibited by law. This policy governs all aspects of employment, including selection, job assignment, compensation, discipline, termination and access to benefits and training.

Inotiv is an Equal Opportunity Employer. It is our policy to provide a fair and equal employment opportunity to all persons, regardless of age, race, color, religion, sex, gender, gender identity, gender expression, sexual orientation, genetic information, disability, national origin, veteran status, or any other basis prohibited by law. This policy governs all aspects of employment, including selection, job assignment, compensation, discipline, termination and access to benefits and training.

At Inotiv, our work helps advance the development of new medicines and therapies that can improve people's lives. As a leading global contract research organization (CRO), we partner with researchers to tackle complex scientific challenges, drive innovation, and help build a healthier, safer world. Bringing together scientists, researchers, technical experts, and business professionals, we provide the expertise and collaboration needed to move research forward. Our people make this work possible, and we value the knowledge, ideas, and contributions each person brings to the team. Whether you are starting your career or bringing years of experience, Inotiv offers opportunities to learn, grow, and play a meaningful role in advancing treatments for patients around the world.

At Inotiv, our people are our greatest asset. We are committed to equality, opportunity, and diversity, and treating our colleagues with the dignity and respect they deserve. We strive to ensure that our people achieve their full potential, and that they enjoy a rewarding career with us.