B-Cell Sequencing & Antibody Discovery Scientist

The Phage; Northern (KY); Hybrid; USD 90,000 - 120,000; Full time

Job summary

Elanco Animal Health is seeking a Research Scientist to drive antibody discovery through advanced single B-cell sequencing and immune library construction. You will isolate antigen-specific B cells, rescue heavy/light chain sequences and push high-throughput NGS workflows for library characterization.

You will collaborate with discovery teams, interpret complex datasets and communicate findings to stakeholders, advancing biologics development while maintaining rigorous documentation and project

Qualifications

• PhD in Immunology, Molecular Biology, Biotechnology or a closely related discipline.
• 0-3 years of relevant industrial experience in biologics discovery.
• Hands-on experience in: Single B-cell sequencing workflows.
• High-speed flow cytometry/FACS.
• NGS library preparation.
• Immune library construction
• Phage display or yeast display technologies
• High-throughput PCR
• Molecular cloning
• Antibody discovery workflows
• NGS data analysis and interpretation.

Responsibilities

• Lead and execute end-to-end single B-cell antibody discovery workflows.
• Process immune cells and perform FACS-based isolation of antigen-specific B cells.
• Perform single-cell sequencing and high-throughput molecular rescue of paired antibody variable-region sequences.
• Contribute to identification and characterization of promising antibody candidates.
• Design and construct high-diversity immune libraries from immunized animals.
• Develop and implement NGS workflows for library characterization.
• Monitor library diversity and track selection dynamics across display platforms.
• Develop and manage panning and selection strategies using phage display or yeast display.
• Identify rare, high-affinity antibody binders from diverse immune repertoires.
• Evaluate and optimize display-based antibody discovery workflows.
• Perform high-throughput RT-PCR and nested PCR.
• Rescue heavy- and light-chain variable regions from individual B cells.
• Apply specialized molecular cloning techniques for antibody discovery workflows.
• Collaborate with bioinformatics teams to integrate large-scale NGS datasets.
• Analyze antibody repertoires and identify novel antibody lineages.
• Evaluate repertoire convergence and selection dynamics.
• Represent the antibody discovery group in multidisciplinary project teams.
• Communicate discovery strategies, experimental results and technical recommendations to stakeholders.
• Collaborate with scientists across discovery research and related functions.
• Maintain accurate electronic laboratory notebooks and research documentation.
• Present technical findings at internal research forums and stakeholder meetings.
• Contribute to scientific decision-making through clear interpretation of discovery data.
• Evaluate emerging antibody discovery technologies.
• Implement improved workflows to increase throughput, repertoire diversity and functional screening capabilities.
• Contribute to reducing discovery timelines and improving biologics development strategies.

Skills

Single B-cell sequencing

FACS

Immune library construction

Antibody discovery workflows

NGS data analysis

Molecular cloning

Phage display

Yeast display

PCR

NGS library preparation

Team collaboration

Education

PhD in Immunology, Molecular Biology, Biotechnology or a closely related discipline

Tools

FACS equipment

NGS platforms

Phage display systems

Yeast display systems

PCR machines

Molecular cloning kits

Job description

Elanco Animal Health is seeking a Research Scientist to drive antibody discovery through advanced single B-cell sequencing and immune library construction. You will isolate antigen-specific B cells, rescue heavy/light chain sequences and push high-throughput NGS workflows for library characterization.

You will collaborate with discovery teams, interpret complex datasets and communicate findings to stakeholders, advancing biologics development while maintaining rigorous documentation and project