Assistant Scientist - iPSC & Neuronal Research

Doc PersonalBeratung GmbH; Columbia (SC); On-site; USD 65,000 - 90,000; Full time

Job summary

The University of South Carolina in Columbia seeks an Assistant Research Scientist to establish and maintain iPSC, neuronal, and brain organoid cultures for comparative studies of hibernation biology.

The position will reprogram somatic cells, optimize differentiation protocols, and perform cellular and molecular experiments using scRNA-seq, immunohistochemistry, microscopy, Western blotting, and functional assays.

Qualifications

• Expertise in induced pluripotent stem cell (iPSC) culture, maintenance, and experimental manipulation.
• Experience with neuronal differentiation and stem cell-based models of neurological or neurodegenerative disease.
• Experience and knowledge of tau biology, including molecular and cellular approaches used to study tau-related processes.
• Experience with relevant molecular and cellular techniques, including immunohistochemistry, fluorescence microscopy, Western blotting, cell-based functional assays, and gene expression analysis.
• Knowledge of experimental design, data analysis, troubleshooting, and optimization of cell culture and molecular biology protocols.
• Experience with single-cell RNA sequencing or other transcriptomic approaches is desirable.
• Ability to train and mentor undergraduate and graduate researchers in laboratory methods and experimental procedures.
• Strong scientific writing and oral communication skills, including preparation of manuscripts, reports, and research presentations.
• Documented evidence of peer-reviewed publications in stem cell biology, neuroscience, neurodegeneration, tau biology, or related fields.

Responsibilities

• Establish and maintain iPSC, neuronal, and brain organoid cultures for comparative studies of hibernation biology.
• Reprogram somatic cells from multiple species, optimize differentiation protocols and conduct cellular/molecular experiments.
• Perform single-cell RNA sequencing, immunohistochemistry, fluorescence microscopy, Western blotting, and cell-based functional assays.
• Contribute to experimental design, data collection and analysis, protocol development and optimization, and training of students.
• Prepare manuscripts and presentations and contribute to grant reports.

Skills

iPSC culture

Neuronal differentiation

tau biology

Immunohistochemistry

Fluorescence microscopy

Western blotting

Gene expression analysis

Single-cell RNA sequencing

Experimental design

Data analysis

Mentoring

Scientific writing

Oral communication

Publications

Education

PhD in Biology/Neuroscience/Cell & Molecular Biology

Tools

Immunohistochemistry

Fluorescence microscopy

Western blotting

Single-cell RNA sequencing

Gene expression analysis

Job description

The University of South Carolina in Columbia seeks an Assistant Research Scientist to establish and maintain iPSC, neuronal, and brain organoid cultures for comparative studies of hibernation biology.

The position will reprogram somatic cells, optimize differentiation protocols, and perform cellular and molecular experiments using scRNA-seq, immunohistochemistry, microscopy, Western blotting, and functional assays.