Research Assistant (Metabolic Liver Disease Lab)

National University of Singapore; Singapore; Presencial; SGD 36 000 - 48 000; Tempo integral

Resumo da oferta

The National University of Singapore invites applications for Research Assistant for the Metabolic Liver Disease Lab in the Department of Medicine, Yong Loo Lin School of Medicine. The RA will contribute to experimental work using functional genomic methods to understand MAFLD mechanisms and to collaborate with pathologists, hepatologists, and data scientists.

The ideal candidate has experience with in vitro mammalian cell-based assays, 2D/3D cell cultures, and molecular biology techniques such

Qualificações

• Minimum education with B.Sc. and/or M.Sc in Cell Biology, Biochemistry, Molecular Biology, Biomedical or related disciplines with research experience.
• Understanding of the molecular/cellular basis of MAFLD, obesity and T2D, e.g., lipid metabolism dysregulation, insulin resistance, inflammatory pathways.
• Experience handling 2D and 3D cell cultures such as hepatocytes, adipocytes or skeletal muscle cells is essential.
• Basic Molecular Biology skills (RNA/protein extraction, qPCR, western blotting) is essential.
• Experience with gene perturbation tools, such as siRNA, shRNA and CRISPR/Cas9 is a strong plus.
• Excellent writing and communication skills in English.
• Ability to coordinate and work in a multidisciplinary team.

Responsabilidades

• Assist in developing, optimizing, and validating in vitro mammalian cell-based assays related to metabolic diseases.
• Perform data analysis, ensure data validity, maintain records, and present findings to colleagues.
• Assist in daily lab management tasks such as inventory, media preparation, autoclaving, and ordering.
• Assist in laboratory equipment management.
• Assist PI/RF with ethics/grant applications.

Conhecimentos

In vitro cell-based assays

2D cell culture

3D cell culture

Hepatocytes

Adipocytes

Skeletal muscle cells

RNA extraction

Protein extraction

qPCR

Western blotting

CRISPR/Cas9

siRNA/shRNA

Excellent English writing

Multidisciplinary teamwork

Formação académica

B.Sc. in Cell Biology or related

M.Sc. in related discipline (preferred)

Descrição da oferta de emprego

Company description

The National University of Singapore is the national research university of Singapore. Founded in 1905 as the Straits Settlements and the Federated Malay States Government Medical School, NUS is the oldest higher education institution in Singapore.

Job description

Purpose of the post

The National University of Singapore invites applications for Research Assistant for the Metabolic Liver Disease Lab in the Department of Medicine, Yong Loo Lin School of Medicine. This role explores functional genomic methods to better understand the molecular mechanisms of metabolic dysfunction-associated fatty liver disease (MAFLD). The RA will get opportunity to collaborate with various teams across the School of Medicine, including pathologists, hepatologists, and data scientists. The ideal candidate will have demonstrated expertise in in vitro mammalian cell-based assays and a keen interest in uncovering the underpinning biological mechanisms of disease.

Responsibilities

• Assist PI/PF to develop, optimize, and validate in vitro mammalian cell-based assays that are related to metabolic diseases (such us cell culture, RNA/protein extraction qPCR, western blotting and other metabolic-related assays)

• To perform other related duties, incidental to the work described herein (such as conduct data analysis, ensure the validity and reliability of data, maintain accurate and complete records of all findings, present findings to colleagues)

• Assist in Daily lab management tasks such as inventory of supplies and samples, preparing sample processing media and solutions, autoclaving materials, ordering supplies and stocks
• Assist in Laboratory equipment management.
• Assist PI/RF with ethics/grant application

Qualifications

• Minimal education with B.Sc. and/or M.Sc. in Cell Biology, Biochemistry, Molecular Biology, Biomedical or related disciplines with research experience.

• Understanding of the molecular/cellular basis of MAFLD, obesity and T2D, e.g., lipid metabolism dysregulation, insulin resistance, inflammatory pathways) are desired.

• Experience in handling 2D and 3D cell cultures such as hepatocytes, adipocytes or skeletal muscle cells is essential.

• Basic Molecular Biology skills (RNA/protein extraction, qPCR, western blotting) is essential.

• Experience with gene perturbation tools, such as siRNA, shRNA and CRISPR/Cas9 is a strong plus.

• Excellent writing and communication skills in English.
• Highly organized and ability to independent judgement and produce a quality work product within tight time constraint.

• Ability to coordinate and work in a multidisciplinary team.