Bioprocess Engineer (Scale-Up)

Palico Biotech Pte Ltd; Singapore; Vor Ort; SGD 80.000 - 120.000; Vollzeit

Benefits dieser Stelle

Competitive salary and equity packages

Work in Singapore’s Biopolis

Zusammenfassung

Palico Biotech Pte Ltd is seeking a Bioprocess Engineer to lead the genetic architecture of the OmniCell™ platform. You will engineer “off-the-shelf” mesenchymal stem cells (MSCs) and oversee multiplexed gene-editing strategies to ensure their therapeutic potency.

Responsibilities include leading CRISPR protocol development, conducting flow cytometry, and supervising a team. Ideal candidates hold a PhD in a relevant field and have over 5 years of experience in mammalian cell engineering.

Qualifikationen

• 5+ years of hands-on experience in mammalian cell engineering.
• Expertise in non-viral delivery methods (electroporation/nanoparticles).
• Deep understanding of the biology of MSCs.

Aufgaben

• Lead the development of CRISPR-Cas12b and base-editing protocols.
• Conduct high-throughput flow cytometry and NGS to verify edit efficiency.
• Supervise a team of junior researchers and lab technicians.

Kenntnisse

Mammalian cell engineering

CRISPR-Cas12b

Bioinformatics tools

Flow cytometry

NGS (Next-Generation Sequencing)

Ausbildung

PhD in Molecular Biology, Immunology, or Cell Biology

Tools

Liquid handling systems

Jobbeschreibung

As a Bioprocess Engineer at Palico Biotech, you will lead the genetic architecture of our OmniCell™ platform. Your primary objective is to engineer “off-the-shelf” mesenchymal stem cells (MSCs) that remain invisible to the host immune system. You will oversee the design and execution of multiplexed gene-editing strategies to silence MHC/HLA markers while ensuring the long-term genomic stability and therapeutic potency of the modified cells.

Key Responsibilities

• Genetic Design: Lead the development of CRISPR-Cas12b and base-editing protocols for silencing HLA Class I and II molecules.
• Validation: Conduct high-throughput flow cytometry and NGS (Next-Generation Sequencing) to verify edit efficiency and off-target profiles.
• Functional Assays: Design in vitro T-cell provocation assays to validate the immune-evasive properties of engineered lines.
• Team Leadership: Supervise a team of junior researchers and lab technicians, ensuring adherence to project timelines and safety protocols.
• IP Documentation: Draft technical reports and patents to secure Palico’s proprietary genetic modifications.

Required Qualifications

• Education: PhD in Molecular Biology, Immunology, or Cell Biology.
• Technical Expertise: Minimum 5 years of hands-on experience in mammalian cell engineering. Expertise in non-viral delivery methods (electroporation/nanoparticles) is highly preferred.
• Industry Knowledge: Deep understanding of the biology of MSCs and the mechanisms of T-cell-mediated rejection.
• Analytical Skills: Proficiency in bioinformatics tools for genomic analysis and experience with automated liquid handling systems.

Why Join Palico?

• Impact: Directly contribute to a platform that eliminates medical waiting lists for critical therapies.
• Ecosystem: Work at the heart of Singapore’s Biopolis, surrounded by world-class infrastructure and collaborative research institutes.
• Growth: Competitive salary and equity packages in a Series B startup with a clear path to global commercialization.

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