Single-Cell Ribo-Perturb-Seq Scientist

ORFeus doctoral network; Utrecht; On-site; EUR 36,000 - 45,000; Full time

Benefits offered by this job

Pension

Paid leave

Relocation support

Secondment

Job summary

The Hubrecht Institute in Utrecht invites applications for a PhD position within the ORFeus network to study translation regulation at single-cell resolution using CRISPR-based smORF editing and single-cell ribosome profiling in organoid models.

You will join a world-class supervisory team, participate in secondments, and enroll in Utrecht University’s doctoral program; the role includes 36 months full-time funded employment under MSCA with mobility and family allowances.

Qualifications

• Master's degree (or equivalent) in life sciences or related field.
• Hands-on experience with molecular biology techniques (CRISPR editing, sequencing).
• Desirable: single-cell methods, ribosome profiling, organoid culture, data analysis (R/Python).
• Good written and spoken English.
• Willingness to travel for secondment and network events.

Responsibilities

• Adapt Perturb-seq vector designs to detect guide RNAs and translation profiles in single cells.
• Validate ribo-perturb-seq using smORF variants across cell lines.
• Apply technology to organoid models and coordinate designs with other DCs.
• Collaborate across the ORFeus network and contribute data to the ORFeome platform.

Job description

The Hubrecht Institute in Utrecht invites applications for a PhD position within the ORFeus network to study translation regulation at single-cell resolution using CRISPR-based smORF editing and single-cell ribosome profiling in organoid models.

You will join a world-class supervisory team, participate in secondments, and enroll in Utrecht University’s doctoral program; the role includes 36 months full-time funded employment under MSCA with mobility and family allowances.