Molecular Cell Biology Technician - CRISPR & Microscopy

KNAW (Nederlandse Akademie van Wetenschappen); Utrecht; On-site; EUR 36,000 - 46,000; Full time

Benefits offered by this job

8% vacation allowance

8.3% year-end bonus

Travel allowance

Internet allowance

Home working allowance

ABP pension

Job summary

The Hubrecht Institute in Utrecht is seeking a Research Technician in Molecular Cell Biology to join the Kops lab. You will support projects involving state-of-the-art cell biology, fluorescence microscopy, and sequencing-based approaches, generating and maintaining human cell lines under supervision in a collaborative, international setup.

Minimum 38 hours per week with a 1-year contract. Applicants should have HBO or Bachelor's degree in a related field and experience with mammalian cell

Qualifications

• Completed HBO or Bachelor's degree in a related field.
• Experience with mammalian cell culture and molecular biology techniques.
• Good organization, independence and teamwork ability.
• Strong English communication skills.

Responsibilities

• Maintain and culture mammalian cell lines under sterile conditions.
• Generate genetically modified cell lines using CRISPR/Cas9 genome editing.
• Produce lentiviral vectors and perform transductions.
• Design and construct plasmids using standard cloning techniques.
• Isolate DNA, RNA, and proteins from mammalian cells.
• Perform PCR, qPCR, and genotyping assays.
• Assist with fluorescence microscopy and NGS library prep.
• Maintain accurate lab records and help organize the lab.

Skills

Mammalian cell culture

Molecular biology techniques

Organizational skills

Team collaboration

English communication

Education

Bachelor's or HBO in Molecular Biology/Cell Biology/Biomedical Sciences

Tools

CRISPR/Cas9 genome editing

Lentiviral vectors

Molecular cloning

PCR/qPCR

Genotyping

Fluorescence microscopy

NGS library preparation

Job description

The Hubrecht Institute in Utrecht is seeking a Research Technician in Molecular Cell Biology to join the Kops lab. You will support projects involving state-of-the-art cell biology, fluorescence microscopy, and sequencing-based approaches, generating and maintaining human cell lines under supervision in a collaborative, international setup.

Minimum 38 hours per week with a 1-year contract. Applicants should have HBO or Bachelor's degree in a related field and experience with mammalian cell