PhD: CRISPR Screen for Cancer Antigen Regulators (smORFs)

ORFeus doctoral network; Amsterdam; On-site; EUR 46,000 - 56,000; Full time

Benefits offered by this job

MSCA funding

PhD enrollment

International collaboration

Competitive salary

Job summary

The Netherlands Cancer Institute in Amsterdam seeks a PhD candidate for a 36-month fully funded MSCA research position. The project identifies regulators of smORF-derived cancer antigen presentation using pooled CRISPR screening and a novel dual-reporter system, integrating interfaces with immunology and cancer biology.

The candidate will undertake a three-month secondment at MyNeo in Ghent (Belgium) and enroll as a PhD candidate at Erasmus University Rotterdam, joining a collaborative ORFeus

Qualifications

• Master's degree in molecular biology, biochemistry, or related life-science field.
• Hands-on mammalian cell culture and basic molecular biology techniques.
• Desirable: experience with CRISPR screening, immunology or mass spectrometry.

Responsibilities

• Develop and deploy a dual-reporter system for smORF translation and HLA surface presentation.
• Screen regulatory factors across translation and antigen-processing pathways.
• Validate top candidates using immunopeptidomics and patient-derived models.
• Collaborate across the ORFeus network and contribute to the regulatory map.

Skills

Mammalian cell culture

Molecular biology

CRISPR screening

Flow cytometry

Immunopeptidomics

Data analysis (high-throughput)

English communication

Education

Master's degree in molecular biology/life sciences

Tools

CRISPR libraries

Mass spectrometry

Flow cytometry equipment

Job description

The Netherlands Cancer Institute in Amsterdam seeks a PhD candidate for a 36-month fully funded MSCA research position. The project identifies regulators of smORF-derived cancer antigen presentation using pooled CRISPR screening and a novel dual-reporter system, integrating interfaces with immunology and cancer biology.

The candidate will undertake a three-month secondment at MyNeo in Ghent (Belgium) and enroll as a PhD candidate at Erasmus University Rotterdam, joining a collaborative ORFeus