Biocatalysis Scientist

AKIN; Delft; On-site; EUR 60,000 - 90,000; Full time

Benefits offered by this job

Stock option plan

Job summary

AKIN is seeking a biocatalysis scientist to own enzyme performance at benchtop scale in Delft. You will develop assays and run studies showing enzyme variants on rubber substrates, collaborating with a chemical engineer to integrate processes and optimize product recovery.

The role includes small-scale in-house enzyme production and day-to-day lab coordination. You will work with external partners for analytics and scale-up, contribute to the R&D roadmap, and ensure data quality for iterative

Qualifications

• Hands-on assays with enzyme activity, kinetics and optimisation.
• Experience expressing/purifying recombinant proteins in bacterial/fungal hosts.
• Structured experimental mindset, DoE, clean data and rapid iteration.

Responsibilities

• Own enzyme performance on rubber substrates and run robust assays.
• Screen enzyme variants and provide feedback for design rounds.
• Connect reaction conditions to product profiles with process developers.
• Manage lab samples, qualifications of batches, and external analytics.
• Maintain cell banks and perform small-scale production for screening.
• Coordinate lab operations and ensure safety/compliance.
• Contribute to R&D roadmap, IP filings and grant reporting.

Skills

Enzyme activity assays

Kinetics

Reaction optimisation

Benchtop bioreactors

DoE design of experiments

Lab coordination

Data handling

English communication

Education

MSc in biochemistry / biotechnology / life science & technology, or related, 3+ years experience

PhD (optional)

Tools

SDS-PAGE

HPLC

UV-Vis spectroscopy

Benchtop bioreactors

Job description

The Role

We are looking for a biocatalysis scientist to own enzyme performance at benchtop scale. You will develop the assays and run the reaction studies that show how our enzyme variants perform on rubber substrates, working side by side with our chemical engineer, who leads the chemistry and product recovery. Enzyme engineering, scale-up production and specialised analytics are carried out with expert partners; your role is to make the enzymes work in our process, keep the feedback loop with those partners sharp, and produce enzyme in-house at small scale. You will also coordinate the day-to-day running of our lab.

Key Responsibilities

• Enzyme performance ownership: developing robust activity assays and running reaction studies on rubber substrates, covering kinetics, stability and the effect of temperature, pH, enzyme loading and co-solvents.
• Variant screening: testing enzyme variants from our engineering partner and turning the results into clear, decision-ready feedback for the next design round.
• Integrated process development: working with our chemical engineer to connect reaction conditions to product profiles, so that upstream and downstream are developed together.
• Partner interface: specifying enzyme batches and checking incoming lots with our scale-up partner, and preparing samples for and interpreting results from external analytical and materials-testing facilities.
• Small-scale enzyme production: maintaining cell banks and expressing and purifying enzyme variants, from shake flasks to our 2.5 L benchtop bioreactors, in our ML-I lab space, so experiments are always supplied.
• Lab coordination (around 10% of your time): inventory, ordering, equipment upkeep, ML-I records, and keeping lab safety and house rules on track.
• Strategic input: contributing to the R&D roadmap, IP filings and grant reporting.

Your First Year

• Reliable, reproducible activity and stability assays for our enzymes on rubber substrates.
• In-house small-scale enzyme supply up and running for screening.
• A well-run screening loop with our enzyme engineering partner, with each round guided by your data.

We Bring

• A fully equipped lab in Delft designed specifically for this programme, access to ML-I lab space, and broad enzyme engineering, scale-up and analytical coverage through partner and service agreements.
• A company that is scaling, with ample room to grow, take on new projects, and influence its development direction.
• Work that pushes the state of the art while remaining critical to the company's commercial success.
• The opportunity to make a direct impact on sustainability in the rubber and tyre industries.
• A flexible work environment that focuses on what empowers you but also expects personal accountability.
• A place where employees are valued regardless of background, beliefs, or identity.
• A competitive compensation package, including stock option plan.

You Bring

• MSc in biochemistry, biotechnology, life science & technology, or a closely related discipline, with at least 3 years of hands-on experience; or a PhD.
• Hands-on experience with enzyme activity assays, kinetics and reaction optimisation.
• Practical experience expressing and purifying recombinant proteins in bacterial and fungal hosts: cell banking, shake-flask and benchtop bioreactor cultures, affinity purification and SDS-PAGE.
• A structured experimental mindset: able to design controlled benchtop studies (including DoE), keep clean, reproducible data, and iterate quickly.
• Organised and comfortable working with external partners and several priorities at once, including the day-to-day running of a shared lab.
• Fluent English. Dutch is not required.

Advantageous

None of these is a strict requirement.

• Experience with enzymes acting on solid, insoluble or polymer substrates (for example plastic- or cellulose-degrading enzymes)
• Experience operating benchtop stirred-tank bioreactors, ideally Getinge (Applikon) systems
• Experience working in ML-I (GMO contained-use) laboratories
• Lab coordination or lab safety contact experience
• Analytical methods such as HPLC and UV-Vis spectroscopy
• Enzyme immobilisation
• Early-stage startup or CRO experience

Terms of the role

• Expected start: mid-January 2027. 80% on-site in Delft.
• Visa sponsorship: We are not able to offer visa sponsorship in the short term.
• Work authorisation: Applications are limited to candidates who already hold Dutch work authorisation or who are eligible for the orientation year permit for recent graduates.