RESEARCH ASSISTANT POSITION AT SR-TIGET IMMUNE CORE at Ospedale San Raffaele

Ospedale San Raffaele; Milano; On-site; EUR 32,000 - 45,000; Full time

Job summary

IRCCS San Raffaele Scientific Institute in Milan, Italy, seeks a Research Assistant for the SR-Tiget Immune Core. Join a dynamic team investigating immunological aspects of gene and cell therapies for genetic diseases. Role covers T/B cell assays, flow cytometry, mouse work, and assay development in an international setting.

The position requires a biomedical degree or PhD, strong lab experience, and excellence in English. One-year contract with opportunities for renewal, based on performance.

Qualifications

• Biomedical degree or PhD with strong lab experience.
• Experience in T cell functional immune assays is required.
• Experience in B cell functional immune assays is a plus.
• Experience in animal handling is desirable.
• Good oral and written English skills are essential.

Responsibilities

• Perform assays to investigate antigen-specific cellular responses (primary cells from disease models).
• Establish antigen-specific T cell lines.
• Differentiate dendritic cells and use for antigen presentation in vitro and in vivo.
• LV mediated modification of DC, B and T cells for experiments.
• Quantify cytokines via multiplex assay (Luminex).
• Determine antibody titers by ELISA.
• Perform basic molecular biology (PCR, cloning, western blots).
• Operate and analyze flow cytometry (FACS sorting).
• Develop and optimize new assays.
• Conduct mouse work (handling, in vivo treatments).
• Train new lab members.

Skills

T cell assays

B cell assays

Animal handling

English communication

Attention to detail

Education

Biomedical degree

PhD

Tools

Flow cytometry

ELISA

PCR

Western blot

Luminex

Job description

IRCCS San Raffaele Scientific Institute in Milan, Italy, is looking for a Research Assistant to join the San Raffaele Telethon Institute for Gene Therapy (SR-Tiget) Immune Core. We are looking for a highly motivated Research Assistant with a strong background in immunological assays, and expertise in experimental animal models to work as part of an enthusiastic and dynamic research team investigating multiple immunological aspects associated to in vivo and ex vivo gene and cell therapies for inherited genetic diseases.

Location: Milano, LOM, IT.

Main activities

• Assays to investigate antigen (Ag)-specific cellular responses (primary cells from several disease models)
• Establishment of Ag-specific T cell lines
• Differentiation of dendritic cells (DC) and their use for Ag presentation in vitro and in vivo experiments
• LV mediated modification of DC, B and T cells for in vitro and in vivo experiments
• Cytokine quantification (Multiplex assay-Luminex)
• Determination of Ag-specific antibodies (Abs) titer (ELISA)
• Basic Molecular biology and biochemistry (PCR, cloning, western blots, enzymatic activity test)
• Flow cytometry (sample preparation/acquisition/analysis, FACS sorting)
• Development and optimization of new assays
• Mouse work (mice handling, in vivo treatments)
• Training of new lab members

Who we are looking for

• Biomedical degree or PhD with strong lab experience
• High accuracy, reliability, precision
• Experience in T cell functional immune assays
• Experience in B cell functional immune assays will be a plus in the selection
• Experience in animal handling
• High motivation to work in an international, fast-paced environment
• Excellent organization skills
• Friendly, collaborative, pro-active mindset
• Good oral and written English skills

We offer

• One-year fixed-term contract (assunzione a tempo determinato), renewable
• Competitive salary, based on qualification and experience
• A dynamic lab environment and the chance to make important contribution to biomedical research

At San Raffaele Scientific Institute, a world-renowned center of excellence for clinical and scientific activities, we believe that considering diversity and promoting an open and inclusive environment is crucial to our success. We are committed to ensure that equal employment opportunities are provided and based on merit without regard to age, religion, sex, disability, medical condition, sexual orientation, cultural and social backgrounds, gender identity or expression, national or ethnic origin, or any other legally recognized status entitled to protection under applicable laws.

We offer attractive conditions and rewards appropriate to an internationally competitive research organization.