Electron Microscopy (EM) Specialist at Human Technopole (Milan, ITALY)

Instruct-ERIC; Milano; In loco; EUR 50.000 - 70.000; Tempo pieno

Descrizione del lavoro

A national facility for structural biology in Milan seeks an Electron Microscopy Specialist. The role involves maintaining the Cryo-Electron Microscopy Unit, supporting scientific collaborations, and aiding users with a focus on advanced imaging techniques. Candidates should have expertise in Cryo-EM and its associated software. This initial three-year contract is renewable, contingent on successful performance.

Competenze

• Experience in Cryo-Electron Microscopy and related techniques.
• Proficient in sample preparation and imaging techniques.
• Familiarity with associated software and computing resources.

Mansioni

• Participate in maintaining the national facility.
• Engage in scientific collaborations.
• Support internal and external users in various applications.

Conoscenze

Cryo-Electron Microscopy

Sample Preparation

Imaging Techniques

Software (SerialEM, Thermo Scientific EPU)

Strumenti

Thermo Scientific Titan Krios G4i

Leica Stellaris confocal microscope

Thermo Scientific Glacios TEM

Descrizione del lavoro

Jobs in Structural Biology and Related Fields

TheCryo-Electron Microscopy (Cryo-EM) Unit, as part of the National Facility for Structural Biologyat Human Technopole seeks to recruit an Electron Microscopy (EM) Specialistthat will actively participate in maintaining the national facility, take part in scientific collaborations, and support internal and external users.

Key tasks and responsibilities

The activities of the EM Specialist will focus on Single-particle Applications, including sample preparation by negative staining and plunge freezing, imaging by TEM and STEM at room and cryogenic temperatures on both side-entry and autoloader equipped systems, and all related applications and software (e.g. SerialEM and Thermo Scientific EPU, Tomography, Velox and MAPS).

About the Cryo-EM Unit

The Cryo-EM Unit workflows span from sample preparation (i.e. negative staining, plunge freezing, high-pressure freezing, freeze substitution, chemical fixation, resin embedding, carbon coating and metal sputter coater, cryo-ultramicrotomy, and micropatterning) to imaging techniques (i.e. TEM\STEM imaging at room and cryogenic temperatures on side-entry holder systems, widefield and confocal cryo-CLEM imaging, SEM imaging and FIB-milling, TEM\EF-TEM imaging at cryogenic temperatures on autoloader-equipped systems). Currently, the Cryo-EM Unit owns: a 300kV Thermo Scientific Titan Krios G4i TEM with Falcon 4i, Selectris X energy filter, and Volta phase-plate; a 200kV Thermo Scientific Glacios TEM with Falcon 4i, Selectris X energy filter, Volta phase-plate, and micro-ED package; a 300kV Thermo Scientific Spectra S/TEM with CETA 2 with speed enhancement, HAADF, BF and DF detectors; a 120kV Thermo Scientific Talos L120C TEM with Gatan Elsa cryo-holder; a Thermo Scientific Aquilos 2 DualBeam cryo-FIB system with EasyLift system, iFLM, AutoSlice-and-View and AutoTEM modules; a Leica Stellaris confocal microscope with white light laser equipped with cryo-stage for CLEM applications; all ancillary sample preparation equipment including plunge freezing devices (2 Thermo Scientific Vitrobot Mark IV, 1 Leica EM GP2 and 1 manual plunger), glow discharger units (Pelco EasyGlow, Quorum GloQube), plasma cleaners (Gatan Solarus II and Diener PICO), carbon and metal sputter coater, high-pressure freezer, automated freeze substitution device, micropatterning device and ultramicrotome for both resin-embedded and vitrified samples. Our microscopes operate with SerialEM, Thermo Scientific EPU, Tomography, EPU-D, Velox and MAPS. Computing resources for EM-related analyses software packages are also available via VMs and HPC. Expansion of the Cryo-EM Unit with more high-end microscopes and equipment is planned in 2026 with two more high-end systems (i.e. 200 and 300 kV CFEG EFTEM with DED and autoloader) for cryo-TEM.

Number of positions offered:1

Deadline:18 th September 2025

More Info and How to Apply (the HR platform is currently on “overhaul” , please apply through LinkedIn )

Contract offered:Initial fixed-term 3 yearsrenewable to permanent position upon successful performance review