Research Associate: Cytoneme Signaling & Live Imaging

The University of Manchester; Manchester; On-site; GBP 34,000 - 42,000; Full time

Benefits offered by this job

Generous employer contribution pension

29 days annual leave + bank holidays

EV car scheme & ride to work

Job summary

The Manning Lab at The University of Manchester invites you to join a three-year, Wellcome-funded post exploring how cytonemes transmit Notch signals. This role sits within a Discovery Award with Exeter, combining cell culture, live imaging, mouse embryo work and advanced 2D-EM to study developmental signalling.

Strong candidates will have a PhD in developmental or cell biology and proven expertise in live-cell imaging, CRISPR gene editing, immunofluorescence, qPCR, PCR and western blotting.

Qualifications

• Hold a PhD or be due to complete within 6 months in developmental or cell biology.
• Strong knowledge of developmental biology or cell biology.
• Significant experience in live-cell or live-tissue fluorescence imaging and quantitative analysis of microscopy data.
• Experience in cell and molecular biology techniques such as CRISPR gene editing, immunofluorescence, qPCR, PCR, and western blotting.
• Willingness to work with mouse embryos and to complete all required training and competency assessment.

Responsibilities

• Undertaking a programme of research to determine how cytonemes mediate signalling during developmental patterning and cell-fate decisions.
• Developing robust image-analysis and quantitative workflows to extract reproducible biological measurements from microscopy data.
• Managing your own research activity, identifying technical challenges and working with collaborators to develop practical solutions.
• Communicating findings through project meetings, conference presentations and high-quality research publications.

Skills

Live-cell imaging

Quantitative image analysis

CRISPR gene editing

Immunofluorescence

qPCR

PCR

Western blotting

Mouse embryo work

Education

PhD in developmental or cell biology

Job description

The Manning Lab at The University of Manchester invites you to join a three-year, Wellcome-funded post exploring how cytonemes transmit Notch signals. This role sits within a Discovery Award with Exeter, combining cell culture, live imaging, mouse embryo work and advanced 2D-EM to study developmental signalling.

Strong candidates will have a PhD in developmental or cell biology and proven expertise in live-cell imaging, CRISPR gene editing, immunofluorescence, qPCR, PCR and western blotting.