Multiomic Immunology Research Assistant: Lupus Nephritis

National Technician Development Centre; Greater London; Hybrid; GBP 28,000 - 36,000; Full time

Benefits offered by this job

41 days holiday

Relocation scheme

On-site gym

CARE pension

Cycle to work scheme

Season ticket loan

Annual leave purchase

Staff Support Service

Job summary

University College London (UCL) seeks a Research Assistant to join Dr Antonelou's programme studying neutrophil–B‑cell immunometabolic crosstalk in autoimmune kidney disease. You will process samples, perform flow cytometry, and coordinate scRNA-seq workflows in a dynamic, multi-disciplinary team.

You will handle longitudinal lupus nephritis samples, contribute to data analyses, and help prepare manuscripts while maintaining stringent records and adhering to GCP and data protection standards.

Qualifications

• A BSc (or equivalent) in Immunology, Biomedical Sciences, Biochemistry or a related discipline, or equivalent laboratory experience
• Demonstrable hands-on experience in multispectral (spectral) flow cytometry, including panel design, sample staining and data analysis — this is essential for the role
• Experience of human sample processing (e.g. PBMC/neutrophil isolation, cryopreservation) in a research laboratory setting
• Sound understanding of laboratory health & safety and good record-keeping practices
• Excellent written and oral communication skills, with the ability to maintain accurate experimental and sample records
• Ability to work independently and as part of a multidisciplinary team, managing time and competing priorities
• Willingness to work flexibly if circumstances allow, including outside normal hours at short notice, to process time‑sensitive clinical sample

Responsibilities

• Liaise with clinical teams to recruit eligible patients and coordinate timely collection of samples
• Process and cryopreserve neutrophils, PBMCs and B-cells from patient and donor samples
• Perform multispectral flow cytometry immunophenotyping of neutrophil and B-cell subsets
• Set up and support neutrophil–B-cell co-culture and functional assays
• Prepare samples for scRNA-seq (10x Genomics) and coordinate with core sequencing facility
• Support MACSima spatial proteomic staining and imaging of renal biopsy sections
• Maintain audit-ready records of sample acquisition, metadata and storage logs
• Ensure compliance with GCP, Human Tissue Act, UK GDPR and approvals
• Contribute to lab meetings, data presentation and manuscript preparation
• Support training of students joining the team

Skills

Multispectral flow cytometry

Panel design

Data analysis

Independent working

Team collaboration

Education

BSc in Immunology or related discipline

Tools

MACSima platform

10x Genomics

LC-MS/MS

Job description

University College London (UCL) seeks a Research Assistant to join Dr Antonelou's programme studying neutrophil–B‑cell immunometabolic crosstalk in autoimmune kidney disease. You will process samples, perform flow cytometry, and coordinate scRNA-seq workflows in a dynamic, multi-disciplinary team.

You will handle longitudinal lupus nephritis samples, contribute to data analyses, and help prepare manuscripts while maintaining stringent records and adhering to GCP and data protection standards.