Mechanobiology PhD: Cryo-EM of Force-Unfolded Proteins

THE FRANCIS CRICK INSTITUTE LIMITED; Greater London; On-site; GBP 18,000 - 24,000; Full time

Job summary

The Francis Crick Institute Limited invites applications for a Crick Joint PhD project led by Alessandro Costa and Sergi Garcia-Manyes. The project explores mechanically unfolded proteins using single-molecule nanomechanics, microfluidics, and cryo-EM to capture structures of force-stretched proteins and uncover mechanotransduction mechanisms.

Candidates should have a Biochemistry/Biophysics/Physical Chemistry background and an interest in protein mechanics, structure, and mechanobiology.

Qualifications

• Background in Biochemistry, Biophysics or Physical Chemistry.
• Interest in protein structure and mechanobiology.
• Willingness to work with cryo-EM and single-molecule techniques.

Responsibilities

• Characterise mechanical stability of individual protein domains in polyproteins.
• Design experimental pipelines combining cryo-EM grids with microfluidic devices.
• Map relative orientation of domains using in silico reconstitution.
• Integrate EM structures with fluorescence microscopy to study protein complexes.

Skills

Biochemistry background

Biophysics knowledge

Physical Chemistry

Education

Background in Biochemistry, Biophysics or Physical Chemistry

Job description

The Francis Crick Institute Limited invites applications for a Crick Joint PhD project led by Alessandro Costa and Sergi Garcia-Manyes. The project explores mechanically unfolded proteins using single-molecule nanomechanics, microfluidics, and cryo-EM to capture structures of force-stretched proteins and uncover mechanotransduction mechanisms.

Candidates should have a Biochemistry/Biophysics/Physical Chemistry background and an interest in protein mechanics, structure, and mechanobiology.