Study Engineer (F/M) H/F

PSL Université Paris; Paris; On-site; EUR 29,000 - 33,000; Full time

Benefits offered by this job

Institute cafeteria

Reimbursement of transportation fees %

Supplementary health insurance

Job summary

Institut Curie in Paris is seeking a highly motivated scientist to join the Gene Regulation team. The position focuses on CRISPR-based screens and the development of stem cell models to study Polycomb group protein function at multiple biological levels.

You will contribute to biochemical studies, transcriptomic and epigenomic profiling, and collaborative projects while training students and maintaining laboratory operations. The role is a full-time fixed-term contract located in Paris.

Qualifications

• Master’s degree in life sciences or equivalent.
• Expertise in molecular biology (cloning, PCR, RNA purification, protein purification).
• Expertise in cell culture.
• Knowledge of or experience in CRISPR-based methods and/or single-cell RNA-seq.

Responsibilities

• Perform CRISPR-based genetic screens coupled to single cell transcriptomics.
• Engineer stem cell models (iPS, ES) by genome editing and characterize their transcriptomic and epigenomic properties.
• Conduct biochemical studies of Polycomb-associated proteins.
• Participate in routine duties such as equipment maintenance, stock renewal, and training.

Skills

Rigor and scientific integrity

Independent work

Team spirit

Education

Master’s degree in life sciences or equivalent

Job description

Missions

Team “Mechanisms of Repression by Polycomb Group Proteins”

Our research team aims to understand the principles that govern gene regulation by the highly conserved family of chromatin modifiers known as Polycomb proteins, from the biochemical to the cellular and organismal levels. We are affiliated with the Institut Curie Research Center’s Genetics and Developmental Biology Unit (Inserm U934/CNRS UMR3215).

• Perform CRISPR-based genetic screens coupled to single cell transcriptomics.
• Engineer various stem cell models (iPS, ES) by genome editing and characterize their transcriptomic and epigenomic properties.
• Conduct biochemical studies of novel Polycomb-associated proteins.
• Participate in all common tasks such as equipment maintenance, renewing stocks, student training, etc.

Activities

• Cloning
• Library preparation (RNA-seq, CUT&RUN-seq, etc.)
• Protein expression and purification, protein pulldowns, Western blot
• Maintenance and differentiation of various stem/progenitor cells
• FACS sorting
• Proliferation and differentiation assays
• Interpretation, synthesis and reporting of results

Constraints and risks

• Biosafety level 2 laboratory
• Routine chemical risks

Training and experience required

• Master’s degree in life sciences or equivalent
• Expertise in molecular biology (cloning, PCR, RNA purification, protein purification)
• Expertise in cell culture
• Knowledge of or experience in CRISPR-based methods and/or single-cell RNA-seq

Skills required

• Rigor and scientific integrity
• Ability to work independently
• Perseverance and team spirit

Contract information

Type of contract: Fixed-term contract

Starting date:December 2026-January 2027

Working time: Full time (218 days/year)

Annual Gross Salary : from 29240 € to 33013 € according to the current grids

Benefits:Institute cafeteria, reimbursement of transportation fees up to 70%, supplementary health insurance

Location of the position: Paris

Faithful to the legacy of its founder, the Institut Curie is deeply committed to promoting equal opportunities, diversity, and inclusion. Convinced that diversity of backgrounds and experiences contributes to collective performance, we strive to provide everyone with a respectful and equitable working environment and encourage all candidates to apply for our job opportunities.All our opportunities are open to people with disabilities

Contact

[e-mail]