Study Engineer (F/M)

PSL Université Paris; Paris; On-site; EUR 29,000 - 30,000; Full time

Benefits offered by this job

Collective catering

Transportation reimbursement up to 70%

Supplementary health insurance

Job summary

The Curie Institute Research Center in Paris is seeking a motivated Study Engineer (IE) in Biology to contribute to a project on aneuploidy, centromeres, and germline deletions in neuroblastoma. The role focuses on hiPSCs, hiPSC-derived sympathoblasts, and neuroblastoma cell lines to unravel tumor initiation and progression mechanisms.

Responsibilities include maintaining and differentiating hiPSCs, performing transfections and molecular assays (including CRISPR), and analyzing DNA, RNA, and

Qualifications

• Master’s degree in molecular and cellular biology, biotechnology, or related field.
• At least 1–2 years of hands-on mammalian cell culture experience including hiPSC/hESCs.
• Solid background in cellular and molecular biology techniques (PCR, RT-qPCR, western blot, cloning, plasmids).

Responsibilities

• Maintenance and differentiation of hiPSCs and culture of several cell lines.
• Performing transfection experiments and molecular assays (including CRISPR) to modulate and analyze gene expression.
• Conducting DNA, RNA, and protein extraction followed by downstream analyses (PCR, RT-qPCR, western blot).
• Applying molecular biology techniques such as cloning and plasmid manipulation.
• Collaborating closely with researchers to analyze experimental data and contribute to the project progress.

Skills

English fluency

Mammalian cell culture

Transfection techniques

PCR

RT-qPCR

Western blot

Cloning

Plasmids

CRISPR

Education

MSc in molecular and cellular biology or related field

Tools

CRISPR

PCR

RT-qPCR

Western blot

Cloning

Plasmids

Job description

The Curie Institute Research Center

The Institut Curie is a major player in the research and fight against cancer. It consists of a hospital and a Research Center of more than 1000 employees with a strong international representativeness.

The objective of the Curie Institute Research Center is to develop basic research and to use the knowledge produced to improve the diagnosis, prognosis, and therapeutics of cancers as part of the continuum between basic research and innovation serving the patient.

Missions

Laboratory

Institut Curie, CNRS UMR 144 – team Daniele Fachinetti: Molecular Mechanisms of Chromosome Dynamics

Missions

Our team Molecular Mechanisms of Chromosome Dynamics, led by Daniele Fachinetti, investigates the fundamental principles that ensure the faithful transmission of genetic material in mammalian cells. We combine cell biology, genetics, epigenetics, biochemistry, and imaging to understand how centromeres are established, maintained, and function throughout the cell cycle. We also study how their deregulation can lead to genome instability – a key driver of many pathologies, including cancer.

Our team is seeking a motivated Study Engineer (IE) in Biology to contribute to a project investigating the role of aneuploidy, centromeres, and germline deletions in the transition from normal to tumoral states in neuroblastoma. The project focuses on human induced pluripotent stem cells (hiPSCs), hiPSC-derived sympathoblasts, and neuroblastoma cell lines to better understand mechanisms of tumor initiation and progression.

The successful candidate will be responsible for:

• Maintenance and differentiation of hiPSCs and culture of several cell lines.
• Performing transfection experiments and molecular assays (including CRISPR technology) to modulate and analyze gene expression.
• Conducting DNA, RNA, and protein extraction followed by downstream analyses (PCR, RT-qPCR, western blot).
• Applying molecular biology techniques such as cloning and plasmid manipulation.
• Collaborating closely with researchers to analyze experimental data and contribute to the advancement of the project

Profile

Education and competences

• Education: Master’s degree (MSc) in molecular and cellular biology, biotechnology, or a related field.
• Experience: At least 1-2 years of hands-on experience in mammalian cell culture and hiPSC/hESCs (mandatory).
• Solid background in cellular and molecular biology techniques (PCR, RT-qPCR, western blot, cloning, plasmids).

Qualifications

• Good organizational skills, ability to work both independently and in a team.
• Rigor, motivation, and enthusiasm for research.
• Fluency in English

All our opportunities are open to people with disabilities

Contract information

Type of contract: CDD

Working time: full time

Remuneration: from 29240.67 to 30396.75€/year according to the current grids

Benefits: Collective catering, reimbursement of transportation fees up to 70%, supplementary health insurance

Institut Curie is an inclusive, equal opportunity employer

and is dedicated to the highest standards of research integrity.