Stage de Master 2 - Biologie H/F

TN France; Nanterre; Sur place; EUR 25 000 - 35 000; Plein temps

Résumé du poste

An innovative internship opportunity awaits you at a leading research laboratory in Nanterre. This role focuses on the experimental validation of a radiation adverse outcome pathway, providing hands-on experience with cutting-edge bioinformatics tools and laboratory techniques. You will analyze transcriptional profiles and conduct critical experiments that enhance our understanding of ionizing radiation effects on brain function. Ideal candidates will possess a Master's degree in biology or a related field and have a keen interest in scientific research. Join a collaborative team dedicated to advancing radiological protection and risk assessment.

Qualifications

• Experience in cell and molecular biology and bioinformatics.
• Laboratory experience with mammalian cell culture is advantageous.

Responsabilités

• Analyze RNA-seq data of cells exposed to ionizing radiation.
• Conduct experiments measuring cell death and cytokine secretion.

Connaissances

Cell and Molecular Biology

Bioinformatics

Laboratory Techniques

English Proficiency

Formation

Master's Degree in Biology or Related Field

Outils

Immunofluorescence Microscopy

ELISA

RT-qPCR

Western Blotting

Description du poste

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Job Reference:

2902c7b4fe8b

Job Views:

1

Posted:

24.04.2025

Expiry Date:

08.06.2025

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Job Description:

Mission

The goal of this Masters-2 internship project is to perform experimental validation of a radiation adverse outcome pathway (AOP). Specific tasks include:

1. Analysis of transcriptional profiles (RNA-seq) of cells exposed to ionizing radiation.
2. Identify altered cellular pathways and biological functions using RNA-seq data and bioinformatics tools.
3. Relate results to Key Events (KE) of a radiation AOP to assess KE validity.
4. Conduct additional experiments to measure parameters such as cell death, cytokine secretion, and functional decline, using assays like immunofluorescence microscopy, ELISA, RT-qPCR, and western blotting.

The results will enhance understanding of ionizing radiation effects on brain function and inform radiological protection for risk assessment. The work will be performed at the Experimental Radiotoxicology and Radiobiology Laboratory (LRTOX) at Fontenay-aux-Roses, supervised by Dmitry KLOKOV.

Applicants should have experience in cell and molecular biology and bioinformatics. Laboratory experience with mammalian cell culture, immunofluorescence microscopy, PCR, and western blotting is advantageous. Candidates should demonstrate interest in science, motivation, organizational skills, teamwork ability, and proficiency in English is a plus.