Postdoc: Structural Biology & NMR for Antibody PTMs

Sanofi Group; Vitry-sur-Seine; Sur place; EUR 42 000 - 54 000; Plein temps

Résumé du poste

Sanofi in Vitry-sur-Seine is seeking a Postdoctoral Fellow in Structural Biology and NMR spectroscopy to study how post-translational modifications in antibody CDRs affect antigen binding and stability. The role combines experimental structural biology with computational modelling to map PTM effects on Fab structure and recognition.

The project integrates 2D/3D-NMR, HDX-MS, cryo-EM and crystallography with cell-free expression to advance molecule optimization and manufacturing processes.

Qualifications

• PhD in NMR spectroscopy, structural biology, biophysics or a related field.
• Hands‑on protein production including U‑13C, 15N and 2H isotope labelling and specific methyl labelling.
• Proven experience in NMR assignment of high molecular weight proteins (backbone and methyl groups)

Responsabilités

• Produce and characterize samples with different levels of modification by mass spectrometry, SEC, DSC and binding assays.
• Perform 2D-NMR analysis on modified and unmodified mAb/Fab samples to map chemical shift perturbations and identify structural signatures induced by PTMs.
• Produce samples via cell-free expression.
• Structurally characterize the Fab‑antigen interaction by NMR, cryo-EM, crystallography and HDX-MS data, to provide an integrated view of how PTMs modulate binding and stability.
• Design, produce and evaluate mutated Fab constructs to determine the role of residues neighboring the PTMs binding affinity and molecular stability.
• Present findings to cross-functional teams and contribute to scientific publications.

Description du poste

Sanofi in Vitry-sur-Seine is seeking a Postdoctoral Fellow in Structural Biology and NMR spectroscopy to study how post-translational modifications in antibody CDRs affect antigen binding and stability. The role combines experimental structural biology with computational modelling to map PTM effects on Fab structure and recognition.

The project integrates 2D/3D-NMR, HDX-MS, cryo-EM and crystallography with cell-free expression to advance molecule optimization and manufacturing processes.