Post doctoral position (M/F)

CNRS; France; On-site; EUR 35,000 - 45,000; Full time

Job summary

CNRS, La Synapse Dynamique team at ESPCI Paris, seeks a researcher to investigate the effects of artificial KCC2 clustering on chloride regulation in hippocampal neurons. The project combines super-resolution imaging, single-molecule tracking, and electrophysiology in mouse and human tissue.

The successful candidate will work within the Brain Plasticity Unit (UMR 8239) and contribute to understanding GABAergic synapse plasticity, membrane dynamics, and activity patterns in epileptic tissue.

Qualifications

• Experience with electrophysiology techniques (patch-clamp) and single-molecule imaging is essential.
• Strong background in neurobiology and imaging modalities (STORM/PALM) is required.
• Ability to work autonomously and communicate results regularly to the team.

Responsibilities

• Develop super-resolution imaging and single-molecule tracking of KCC2 in interneurons and pyramidal cells.
• Characterize membrane distribution and mobility of KCC2 across neuron types in mouse and human tissue.
• Perform electrophysiology recordings (patch-clamp EGABA, MEA) in human epileptic tissue slices.

Skills

Neurosciences

Electrophysiology

Super-resolution imaging

Autonomy

Team collaboration

Progress reporting

Job description

Organisation/Company CNRS Department Plasticité du cerveau Research Field Neurosciences Biological sciences Researcher Profile Recognised Researcher (R2) Application Deadline 11 Nov 2026 - 23:59 (UTC) Country France Type of Contract Temporary Job Status Full-time Hours Per Week 35 Offer Starting Date 1 Jan 2027 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No

Offer Description

Effects of artificial KCC2 clustering on intracellular chloride regulation in hippocampal interneurons and pyramidal cells, and on epileptic activity

We will develop super-resolution imaging and single-molecule tracking of KCC2 in interneurons and pyramidal cells in mouse hippocampal slices or human epileptic tissue.
After characterizing and comparing the membrane distribution and mobility of KCC2 in interneurons and pyramidal cells, we will determine the impact of antibody-induced artificial KCC2 clustering.
We will then investigate the functional impact of this strategy using electrophysiology (patch-clamp recordings of EGABA and multielectrode array (MEA) recordings of network activity) in human epileptic tissue slices.

The successful candidate will join the La Synapse Dynamique team, led by Sabine Lévi and based in the Brain Plasticity Unit (UMR 8239) at ESPCI Paris. Our research focuses on the cellular and molecular mechanisms underlying the stabilization of nascent synapses during development and the plasticity of mature synapses, with a particular emphasis on hippocampal GABAergic synapses. We use single-molecule imaging techniques, including single-molecule tracking in live neurons and super-resolution STORM/PALM microscopy.

• Neurosciences
• Electrophysiology and super-resolution imaging
• Autonomy
• Ability to work effectively as part of a team
• Ability to regularly report results and progress to the line manager