Job offer

European Commission; France; Sur place; EUR 30 000 - 45 000; Plein temps

Résumé du poste

L'Institut de Pharmacologie Moléculaire et Cellulaire recherche un chercheur post-doctorant pour un projet collaboratif sur le TMEM33 et son rôle dans la maladie du foie gras. Le poste de 30 mois nécessitera des compétences en biologie lipidique et en expérimentation animale. Ce rôle offre une opportunité unique de contribuer à la recherche innovante sur les complications métaboliques liées à l'obésité dans un environnement dynamique à Nice, France.

Qualifications

• Expérience en biologie des lipides et expérimentations sur souris.
• Intérêt pour la physiologie du foie et les maladies connexes.
• Connaissance de la biologie moléculaire requise.

Responsabilités

• Évaluer le rôle du TMEM33 dans les comorbidités métaboliques associées à l'obésité.
• Utiliser des souris knockout spécifiques pour démontrer les effets du TMEM33.
• Effectuer des études de ciblage thérapeutique avec des nanoparticules.

Connaissances

Mécanismes métaboliques

Physiologie hépatique

Biologie des lipides

Expériences sur animaux

Microscopie confocale

Immunologie

Formation

Doctorat en sciences biologiques ou domaine connexe

Description du poste

Organisation/Company CNRS Department Institut de Pharmacologie Moléculaire et Cellulaire Research Field Biological sciences Researcher Profile Recognised Researcher (R2) Country France Application Deadline 16 Jun 2025 - 00:00 (UTC) Type of Contract Temporary Job Status Full-time Hours Per Week 35 Offer Starting Date 1 Jul 2025 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No

Offer Description

A post-doctoral position (30 months) is currently available for a collaborative project (co-PI: Pr. Aimin XU, The University of Hong Kong, China), focusing on the role of TMEM33 in fatty liver disease. Candidates should have a keen interest in understanding metabolism, liver physiology and associated disease states. A strong backround in lipid biology and mouse experimentation is required.

Transmembrane protein 33 (TMEM33) is an endoplasmic reticulum (ER)-localised membrane with poorly-defined functions. Our preliminary result reveals a progressively increased TMEM33 expression in the liver of obese patients with worsening degree of metabolic dysfunction-associated steatotic liver disease (MASLD; NAFLD). In mice with diet-induced MASLD and dyslipidemia, a marked upregulation of TMEM33 occurs predominantly in parenchymal hepatocytes of the liver, whilst TMEM33 knockout mice are refractory to high fat diet-induced dyslipidemia, MASLD and liver injury. Furthermore, siRNA-mediated knockdown of TMEM33 leads to an obvious attenuation of toxic lipids-induced steatosis in both human and mouse hepatocytes. Using RNA-sequencing, we have identified several metabolic pathways altered by TMEM33 deficiency in the mouse liver, including ER stress-responsive chaperones, lipid and sterol metabolism, and immuno-inflammatory responses. Based on these findings, we propose increased hepatic expression of TMEM33 as an important player in initiating and/or perpetuating obesity-induced metabolic complications such as dyslipidemia and MASLD. In this project, we will further evaluate its pathophysiological role in obesity-associated metabolic comorbidities using hepatocyte-specific TMEM33 knockout mice, and will elucidate the molecular mechanism whereby this ER protein disrupts metabolic homeostasis by identifying the direct downstream effectors and interaction partners of TMEM33 in hepatocytes. Additionally, we plan to conduct a proof-of-concept study to explore the therapeutic potential of nanoparticle-mediated hepatocyte-selective delivery of TMEM33 siRNA for the treatment of aforementioned metabolic disorders. The findings from this study are expected to shed new light on molecular pathways linking ER function and
lipid metabolism in hepatocytes, and to provide scientific foundation for future development of novel hepatocytes-targeted therapeutics for precision management of obesity-related metabolic complications.

Our group takes advantage of a multidisciplinary approach including molecular biology, proteomic profiling, lipidomics, electrophysiology, calcium imaging, and genetic manipulations to investigate the molecular physiology and pharmacology of ion channels and regulators.
Our laboratory is part of the CNRS Institute of Molecular and Cellular Pharmacology located in the City of Nice-Sophia Antipolis (South East of France). This is a leading centre in biology research with state of the art equipment. The city of Nice offers an international dynamic cultural experience combined with a magnificent scenery and easy access to air travel.

1) Animal experimentation
2) Confocal microscopy
3) Immunology
4) Metabolism
5) Lipid biochemistry

Additional comments

Interested candidates should e-mail a letter of application, including a CV and the names and addresses of at least two referees to:
Eric HONORE ([e-mail] )
IPMC-CNRS
Université Côte d'Azur
660, route des Lucioles
Sophia Antipolis
06560 Valbonne, France
Tel : [phone]
http://www.ipmc.cnrs.fr/?page=honore