Job offer

European Commission; France; Sur place; EUR 40 000 - 60 000; Plein temps

Résumé du poste

Un centre de recherche de renommée mondiale recherche un doctorant en immunologie pour explorer la dynamique de stimulation des TCR. Ce poste implique des techniques avancées telles que l'optogénétique, la cytométrie en flux et les études transcriptomiques, offrant une opportunité précieuse dans un environnement collaboratif. Les candidats idéaux posséderont une formation solide en biologie ou immunologie, ainsi qu'un esprit d'équipe.

Qualifications

• Fortes compétences en immunologie et biologie cellulaire.
• Compétences avancées en cytométrie en flux, biophysique et transcriptomique.
• Capacité à produire des protéines recombinantes et à manipuler des modèles murins.

Responsabilités

• Étudier comment la dynamique de stimulation du TCR façonne les réponses T cellulaires.
• Cartographier l'influence des dynamiques de stimulation sur les programmes transcriptionnels des cellules T.
• Évaluer l'impact à long terme des dynamiques de stimulation sur la destinée des cellules T.

Connaissances

Immunologie

Biologie cellulaire

Cytométrie en flux

Biochimie

Transcriptomique

Anglais scientifique

Travail en équipe

Formation

Master ou équivalent

Description du poste

Organisation/Company Centre de Recherche en Cancérologie de Marseille Research Field Biological sciences » Biology Researcher Profile Other Profession Positions PhD Positions Country France Application Deadline 21 Jul 2025 - 00:00 (Europe/Paris) Type of Contract Temporary Job Status Full-time Offer Starting Date 1 Oct 2025 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No

Offer Description

CONTEXT

In the lymph node, T cell activation is initiated by a series of transient interactions with antigen presenting cells that present the cognate ligand for the T cell receptor (TCR) on their surface. To date, the mechanisms that allow T cells to integrate a series of transient TCR stimulations are poorly understood. Furthermore, the impact of dynamic parameters of TCR triggering (duration, frequency) on the outcome of T cell activation remains to be determined.

To address these fundamental questions, we have developed the Light-inducible T cell engager (LiTE) system, an optogenetics-based TCR agonist whose activity is under the control of light. LiTE provides unique spatiotemporal control of TCR triggering in primary T cells. This has allowed us to show that TCR stimulation dynamics influence the outcome of T cell activation.

MISSIONS

Under the supervision of the PI of the project, the PhD candidate will leverage the original LiTE system to investigate in depth a fundamental question in immunology: how the dynamics of TCR stimulation shape T cell responses?

More specifically, using state-of-the-art optogenetics, phospho-proteomics and transcriptomics technologies, The PhD candidate will :

- comprehensively map the influence of TCR stimulation dynamics on the transcriptional programs of primary mouse T cells.

- unravel the encoding of TCR stimulation dynamics in specific intracellular signaling cascades.

- evaluate the long-term influence of TCR stimulation dynamics on T cell fate in relevant physiological models.

Where to apply

E-mail [e-mail]

Requirements

Research Field Biological sciences » Biology Education Level Master Degree or equivalent

Skills/Qualifications

• Strong background in immunology and cell biology

• Advanced skills in flow cytometry, biochemistry and transcriptomics

• Fluency in scientific English (written and oral) required, French is an asset

Specific Requirements

• Ability to produce recombinant proteins in both eukaryotic and prokaryotic systems.

• Ability to handle murine models, purify immune primary cells.

• Team spirit, ability to collaborate with different teams.

Languages ENGLISH Level Good

Languages FRENCH Level Basic

Additional Information

Selection process

We are seeking highly motivated candidates to join our team.

To apply, please submit your application via email. Your application must include the following:

• The email address of a professional reference

Shortlisted candidates will be contacted for an interview as part of the final selection process.