Job offer

European Commission; France; Sur place; EUR 40 000 - 60 000; Plein temps

Résumé du poste

Une institution de recherche en France propose un stage pour un chercheur à premier stade, centré sur l'amélioration de l'imagerie 3P pour des études tissulaires. Le candidat travaillera dans une équipe dynamique, participant activement à des projets innovants sur l'imagerie de tissus profonds avec des techniques avancées de détection et d'optique adaptative.

Qualifications

• Chercheur au premier stade (R1) avec un fort intérêt pour l'imagerie et les techniques de microscopie.
• Expérience en microscopie d'au moins un an souhaitée.
• Capacité à collaborer dans une équipe dynamique.

Responsabilités

• Caractériser les modalités de contraste en microscopie.
• Optimiser la détection des signaux de TSFG et de fluorescence.
• Développer des techniques avancées pour l'imagerie.

Connaissances

Microscopie multiphotonique

Techniques d'imagerie

Analyse de données

Formation

Études en Physique ou domaine connexe

Description du poste

Organisation/Company: CNRS

Department: Laboratoire d'optique et biosciences

Research Field: Physics

Researcher Profile: First Stage Researcher (R1)

Country: France

Application Deadline: 7 Jul 2025 - 23:59 (UTC)

Type of Contract: Temporary

Job Status: Full-time

Hours Per Week: 35

Offer Starting Date: 1 Oct 2025

Funding: Not funded by a EU programme

Research Infrastructure Staff Position: No

Offer Description

The project will take place in the 'Advanced microscopies' group of the Lab for Optics and Biosciences at Ecole Polytechnique (LOB). Our team specializes in multiphoton microscopies and their application to tissue studies. The work involves collaboration with a team of ~4 people within a larger microscopy team (~25 persons) and an active network including IOGS, Amplitude, Inst Vision, Pasteur, INMED.

Two-photon (2P) microscopy uses infrared femtosecond lasers (900 nm) to record fluorescence images with 3D cellular resolution at depths of 300-500 µm in scattering tissues, widely used in neuroscience and embryology. However, imaging at greater depths is challenging due to scattering. Recently, three-photon (3P) excitation using new infrared sources (1300-1700 nm, 50 fs, 1 MHz) has enabled imaging beyond 1 mm depth, opening new research possibilities and technological challenges. The group is pioneering 3P imaging with innovative multibeam laser sources.

The project's goal is to enhance 3P imaging performance by combining contrast modalities and exploring its application in deep tissue microscopy of fish and mouse tissues. The work will focus initially on a label-free contrast modality called third-order sum-frequency generation (TSFG) microscopy, which is sensitive to hemoglobin and useful for analyzing blood flow in vivo. The intern will characterize this contrast, optimize simultaneous detection of TSFG and 3P fluorescence signals, and develop advanced techniques such as adaptive optics for aberration correction and multiplane excitation for faster imaging, for deep and long-term tissue imaging.