Ingénieur d’étude en recombinaison homologue (F/H)

Institut Curie; Paris; Hybrid; EUR 32,000 - 42,000; Full time

Job summary

The Institut Curie in Paris invites applications for an Engineer to join the Compartmentalization and Dynamics of Nuclear Functions team led by Angela Taddei. The project focuses on studying the DDR and genome spatial organization in S.

cerevisiae using advanced imaging and molecular biology approaches. The recruited engineer will generate yeast strains, adapt oligopaint techniques to visualize 3D chromosome conformation, and perform experiments, data analysis, and documentation for regular

Qualifications

• Experience with yeast genetics and strain construction.
• Familiarity with DNA damage response pathways.
• Experience with super-resolution microscopy is a plus.

Responsibilities

• Generate new yeast strains required for the project.
• Adapt oligopaint technique to visualize 3D chromosome conformation during DDR.
• Record experiments, analyze results, and present progress at lab meetings.

Skills

Yeast strain construction

Super-resolution imaging

DNA damage response

Yeast genetics

Oligopaint technique

Tools

Plasmids

Yeast strains

Job description

The laboratory

The successful candidate will join the Compartmentalization and Dynamics of Nuclear Functions team led by Angela Taddei at Institut Curie (https://curie.fr/equipe/taddei). Our research focuses on understanding how nuclear organization regulates genome functions, with a particular emphasis on homologous recombination. We combine state-of-the-art in vivo quantitative microscopy approaches, including super-resolution imaging, with genetic and molecular biology techniques.

The project

This position is part of a project aimed at studying the various stages of the DNA damage response (DDR) in relation to genome spatial organization in the yeast S. cerevisiae.

The missions :

The recruited engineer will have two main responsabilities:

• The individual will strengthen the team’s capacity to generate new yeast strains required for the project’s progress (tool construction: plasmids and strains)
• He/she will adapt the oligopaint tcehnique to vizualize 3D chromosome conformation during the DNA damage response. This part of the project will be carried out with the assistance of a reserach engineer from the Taddei team and a research engineer from the Curie Coretech platform.

As part of his duties, the engineer will conduct experiments, analyzeresults, maintain an up-to-date electronic laboratory notebook, and present project progress at laboratory meetings.