Development of fluorescent probes to determine live cell asymmetric membrane properties in path[...]

Karlstad University; Strasbourg; Sur place; EUR 35 000 - 55 000; Plein temps

Résumé du poste

Une entreprise innovante recherche un chercheur postdoctoral passionné par la biologie moléculaire et la microbiologie. Ce rôle captivant implique le développement de sondes fluorescentes génétiquement encodées pour étudier les propriétés des membranes des bactéries pathogènes. Vous aurez l'opportunité de travailler dans une équipe dynamique et multidisciplinaire, en utilisant des techniques d'imagerie avancées pour mieux comprendre la résistance aux antimicrobiens. Si vous êtes motivé par la recherche de solutions aux problèmes de santé mondiale, cette position est faite pour vous.

Qualifications

• Expérience en biologie moléculaire et microbiologie requise.
• Compétences en microscopie et techniques de fluorescence appréciées.

Responsabilités

• Développer des peptides fluorescents pour quantifier la charge de surface des membranes bactériennes.
• Travailler au sein d'une équipe multidisciplinaire sur des approches moléculaires et cliniques.

Connaissances

Biologie moléculaire

Microbiologie

Microscopie

Techniques de fluorescence

Biophysique des membranes

Chimie analytique

Formation

PhD ou équivalent

Outils

Imagerie de fluorescence

Imagerie par deux photons

Imagerie spectrale

Description du poste

Development of fluorescent probes to determine live cell asymmetric membrane properties in pathogenic bacteria

The Université catholique de Louvain (UCLouvain) is internationally recognized for research and teaching quality in many different fields of expertise.

One of the most pressing problems in health care is the concerning rise of antimicrobial resistances in pathogenic bacteria. Globally, 4.95 million deaths have been associated with antimicrobial resistance in 2019 while the WHO predicts 10 million deaths for 2050. New antibiotic therapy and targets are hence urgently needed to fight this global health problem. Because of their peculiar composition and properties, bacterial cell membranes constitute excellent targets for antibiotic therapy but bacteria developed resistance mechanisms that lead to alterations of membrane properties.

In this project, the postdoctoral researcher will therefore develop genetically encoded fluorescent peptides that simultaneously quantify inner and outer monolayer surface charge in live bacteria. These probes will be calibrated on membrane models such as Giant unilamellar vesicles of known surface charge and lipid composition, and further validated in live bacteria. In a long term these probes should allow us to understand antimicrobial resistance towards last resort cationic peptides and its dynamic development.

This postdoctoral position is funded for 3 years. The candidate will have experience in molecular biology and microbiology. Experience in microscopy, fluorescence techniques, membrane biophysics or analytical chemistry is a bonus. The candidate will be trained in advanced imaging techniques such as fluorescent lifetime imaging, anisotropy imaging, two-photon imaging, spectral imaging and related image analysis and develop a deep understanding in peptide/membrane interactions and related resistance mechanisms.

The postdoc will work in a vibrant, multidisciplinary team that has experience in molecular and clinical approaches. She/He will join the team at the Woluwé-Saint-Lambert campus of UCLouvain University in Brussels, Belgium.

Research Field: Biological sciences » Biological engineering

Education Level: PhD or equivalent

Research Field: Pharmacological sciences » Other

Education Level: PhD or equivalent

Research Field: Physics » Biophysics

Education Level: PhD or equivalent

Skills/Qualifications: Molecular Biology, Microbiology, Experience in microscopy and/or fluorescence spectroscopy, Membrane Biophysics

Specific Requirements: Please send a C.V. and a motivation letter to [e-mail]. Please indicate in the motivation letter how well your qualifications match the position.