Cryo-EM Membrane Protein Scientist

CNRS; France; On-site; EUR 42,000 - 54,000; Full time

Job summary

The CNRS Institut de biologie structurale in Grenoble invites applications for a temporary, full-time researcher to lead cell biology experiments and membrane protein expression using baculoviruses or lentiviruses. The project focuses on solubilisation, purification, and quality control of membrane proteins for cryo-EM structure determination.

You will work with electron microscopy facilities, prepare samples, analyze cryo-EM images, draft reports, manage resources, and stay abreast of

Qualifications

• Expertise in protein production in insect and mammalian cells.
• Expertise in purification of membrane proteins.
• Expertise in biochemical and biophysical techniques for protein characterisation.
• Expertise in cryo-electron microscopy and crystallography techniques.
• Expertise in bioinformatics tools for analysing cryo-electron microscopy images.
• Knowledge of molecular biology techniques (PCR, DNA purification).
• Manage relationships and the organisation of experiments with platform managers and staff at partner institutes.
• Be meticulous and organised.
• Be able to interact with colleagues, line managers and collaborators in an international environment.
• English (written/spoken) at B1 level.

Responsibilities

• Carry out production of membrane proteins in insect and mammalian cells using baculoviruses or lentiviruses.
• Develop and adapt protocols for solubilisation, purification and concentration of membrane proteins.
• Perform biochemical and biophysical quality control of samples.
• Assist with preparation and analysis of samples for cryo-electron microscopy.
• Analyse cryo-EM images to reconstruct protein structures in apo and holo forms.
• Interpret and present results, ensuring follow-up and quality.
• Draft experimental reports and protocols.
• Manage and organise technical resources and biological materials with partner platforms.
• Keep track of developments in the field.

Skills

Protein production

Membrane protein purification

Biochemical techniques

Biophysical techniques

Cryo-EM

Crystallography

Bioinformatics tools

Molecular biology

Project coordination

Attention to detail

Communication

Job description

The CNRS Institut de biologie structurale in Grenoble invites applications for a temporary, full-time researcher to lead cell biology experiments and membrane protein expression using baculoviruses or lentiviruses. The project focuses on solubilisation, purification, and quality control of membrane proteins for cryo-EM structure determination.

You will work with electron microscopy facilities, prepare samples, analyze cryo-EM images, draft reports, manage resources, and stay abreast of