Technical Assistant in T Cell Engineering and Molecular Biology

dkfz. Deutsches Krebsforschungszentrum; Heidelberg; Vor Ort; EUR 42.000 - 62.000; Vollzeit

Benefits dieser Stelle

30 days of vacation per year

Family-friendly working environment

Sustainable travel to work: subsidized

Personal development opportunities

Corporate Health Management Program

Zusammenfassung

dkfz. Deutsches Krebsforschungszentrum in Heidelberg invites applications for a Technical Assistant in T Cell Engineering and Molecular Biology.

The role supports the Clinical Cooperation Unit within Neuroimmunology and Brain Tumor Immunology, focusing on cloning, cell culture, and vector work. As a hands-on team member, you will contribute to laboratory management, experimental execution, and documentation in accordance with biosafety and GenTG/S1–S2.

Qualifikationen

• Completed training as a biological, medical, or chemical technical assistant (BTA/MTA/CTA), a bachelor's degree in a life science subject, or comparable knowledge and experience
• Solid practical experience in molecular cloning
• Experience in sterile mammalian cell culture, ideally also primary cell culture
• Previous experience in an immunology or immunotherapy laboratory is advantageous, e.g., PBMC isolation, REP, Jurkat cell culture, transfection/transduction, FACS, or sequencing of NGS libraries
• Willingness to work with primary human material (blood and tissue samples)
• Careful and complete documentation of experiments, ideally using an electronic lab notebook
• Precision, teamwork, and organizational skills

Aufgaben

• Laboratory management, ordering consumables, onboarding new colleagues, coordinating repairs, annual inventory, etc.
• Molecular cloning, including site-directed mutagenesis of plasmids and Golden Gate assembly
• Generation and sequence verification of expression constructs; bacterial transformation, plasmid preparation, and maintenance of the plasmid and glycerol stock collection
• Maintenance of cell lines (e.g., Jurkat and derivatives)
• Transfection and electroporation of cell lines and primary cells; production and titration of lentiviral/retroviral vectors and transduction of target cells
• Isolation of PBMCs from whole blood and leukapheresis material; activation, expansion, and cryopreservation of primary human T cells, including rapid expansion protocols (REP)
• Documentation of work in accordance with genetic engineering (GenTG/S1–S2) and biosafety requirements
• Supervising students and apprentices

Kenntnisse

Molecular cloning

Sterile mammalian cell culture

PBMC isolation

Transfection/transduction

Documentation

Ausbildung

BTA/MTA/CTA training or life science degree

Tools

Laboratory management tools

Jobbeschreibung

Technical Assistant in T Cell Engineering and Molecular Biology

Reference number: 2026-0181

• Full-time
• Neuroimmunology and Brain Tumor Immunology

The German Cancer Research Center (DKFZ) is one of Europe’s largest cancer research centers. “Research for a life without cancer" is the mission of our world-class scientists and all our team members.

We investigate how cancer develops, identify cancer risk factors and search for new cancer prevention strategies. We develop new methods with which tumors can be diagnosed more precisely and cancer patients can be treated more successfully. Every contribution counts – whether in research, administration or infrastructure. This is what makes our daily work so meaningful and exciting.

TheClinical Cooperation UnitNeuroimmunology and Brain Tumor Immunology (Head: Professor Michael Platten) develops personalized T cell therapies for solid tumors. Our work combines the identification and validation of tumor-reactive T cell receptors, high-throughput molecular cloning and vector engineering, and functional testing of engineered T cells in cellular model systems. We translate these findings into clinically applicable therapeutic candidates together with our clinical and industry partners.

To strengthen our team, we are seeking, as of October 2026, a

Your Tasks

• Laboratory management, ordering consumables, onboarding new colleagues, coordinating repairs, annual inventory, etc.
• Molecular cloning, including site-directed mutagenesis of plasmids and Golden Gate assembly
• Generation and sequence verification of expression constructs; bacterial transformation, plasmid preparation, and maintenance of the plasmid and glycerol stock collection
• Maintenance of cell lines (e.g., Jurkat and derivatives)
• Transfection and electroporation of cell lines and primary cells; production and titration of lentiviral/retroviral vectors and transduction of target cells
• Isolation of PBMCs from whole blood and leukapheresis material; activation, expansion, and cryopreservation of primary human T cells, including rapid expansion protocols (REP)
• Documentation of work in accordance with genetic engineering (GenTG/S1–S2) and biosafety requirements
• Supervising students and apprentices

Your Profile

• Completed training as a biological, medical, or chemical technical assistant (BTA/MTA/CTA), a bachelor's degree in a life science subject, or comparable knowledge and experience
• Prerequisites:
• Solid practical experience in molecular cloning
• Experience in sterile mammalian cell culture, ideally also primary cell culture
• Previous experience in an immunology or immunotherapy laboratory is advantageous, e.g., PBMC isolation, REP, Jurkat cell culture, transfection/transduction, FACS, or previous experience with the generation and sequencing of NGS libraries
• Willingness to work with primary human material (blood and tissue samples)
• Careful and complete documentation of experiments, ideally using an electronic lab notebook
• Precision, commitment, teamwork, and flexibility regarding tasks and approaches, as well as strong coordination and organizational skills

We Offer

30 days of vacation per year

Family-friendly working environment

Sustainable travel to work: subsidized Germany job ticket

Unleash your full potential: targeted offers for your personal development to further develop your talents

Our Corporate Health Management Program offers a holistic approach to your well-being

Duration:The position is initially limited to 2 years.

We are convinced that an innovative research and working environment thrives on the diversity of its employees. Therefore, we welcome applications from talented people, regardless of gender, cultural background, nationality, ethnicity, sexual identity, physical ability, religion and age. People with severe disabilities are given preference if they have the same aptitude.

Notice:We are subject to the regulations of the Infection Protection Act (IfSG). Therefore, all our employees must provide proof of immunity against measles.