Research assistant (f/m/d) (DM-840)

Freie Universität Berlin; Berlin; Vor Ort; EUR 48.000 - 56.000; Vollzeit

Benefits dieser Stelle

Direct supervision

Lab collaboration

Flexible hours

Public transport nearby

Library access

Zusammenfassung

The Freie Universität Berlin invites applications for a doctoral position in the RNA Dynamics group led by Prof. Dr. Lydia Herzel to study transcription dynamics in E.

coli. The project is part of a DFG-funded FOR 6037 and involves characterizing transcription factors and RNA polymerase pausing in relation to RNA processing. Requirements include a completed Master’s degree in biochemistry or a related field, strong motivation, and excellent English communication.

Qualifikationen

• Master's degree in biochemistry or related field.
• Interest in transcription and gene regulation.
• Ability to work independently in day-to-day lab work.
• Excellent documentation and communication in English.

Kenntnisse

Independent working

English communication

Teamwork

Strong motivation

Data analysis

Ausbildung

Master's degree in biochemistry or related field

Tools

Molecular biology techniques

Jobbeschreibung

Who We Are

We are a young group interested in understanding quantitative aspects of gene regulation and coordination of the different steps of gene expression. We study Escherichia coli and the opportunistic fungal pathogen Candida albicans using molecular biology, biochemistry and quantitative biology approaches. Ultimately, we aim to understand how fundamental processes of gene regulation connect to regulation at the organismal level and to pathology, and to make that knowledge useful for biomedicine. We are part of the Department of Biology, Chemistry and Pharmacy at the Institute of Chemistry and Biochemistry and maintain collaborations within FU Berlin, across Germany and internationally.

What You Can Expect In Your New Role

In the research group of Prof. Dr. Lydia Herzel (RNA Dynamics) a doctoral position is to be filled to work on transcription dynamics in E. coli and to characterize the contribution of a number of putative transcription factors, as well as the interconnection of RNA polymerase pausing to RNA processing. This project is part of a DFG-funded research unit FOR 6037 investigating the structure and function of multi-subunit RNA polymerases (msRNAPs) in different organisms, viruses and organelles and a direct collaboration with the structural biochemistry group at the institute.

Key Requirements

A completed Master’s degree (or equivalent) in biochemistry or a comparable field.

Desirable Applicants Should Bring

• Strong interest in the fundamental function and regulation of the transcription process by RNA polymerases
• High motivation to drive this project forward and develop it further
• Ability to work independently in day-to-day lab work and planning
• A reliable, careful working style and good documentation skills
• Interest in combining experiments and data analysis
• Very good oral and written communication skills in English
• Teamwork skills and an interest in scientific exchange

Professional experience (desired for conceiving and performing the experiments and analyses in the proposed project)

• Very good knowledge of the fundamentals of gene expression in bacteria and eukaryotes
• Extensive practical experience in molecular biology, in particular cloning in E. coli on plasmids and into the genome
• Extensive practical experience in microbiology
• Experience with (in vitro) transcription analysis methods
• Practical experience with immunoprecipitation and protein and RNA analysis
• Very careful documentation and presentation of research results
• Experience with high-throughput sequencing methods
• Experience in designing and performing bioinformatic data analyses

Benefits And Other Advantages

• Direct supervision in the lab
• Daily exchange with the group and other labs at the institute
• Thesis advisory committee with experts in the field through integration into the research unit infrastructure
• Annual (peer) exchange as part of the research unit symposium
• Lab placements in groups of the research unit
• Very good data infrastructure
• Participation in biochemistry teaching and the opportunity to gain experience supervising students in the lab
• Modern workplace in the leafy suburb of Dahlem
• Flexible working hours and mobile working by arrangement, where possible
• The compatibility of work and family life
• Numerous training and continuing education options for professional and personal development
• Opportunity to participate in University Sports Center courses and the health promotion program
• Free parking close to the office and good public transportation connections
• Discounted “Job Ticket” for public transportation
• Free use of the Freie Universität library system
• Employee discount in the cafeteria
• Exclusive deals for products and services through Corporate Benefits

You can also get in touch with Mr./Ms. Prof. Dr. Lydia Herzel via phone.: [phone] or e-Mail: [e-mail]