PhD Candidate (f/m/x) – RNA Quality Control Lab

LungResearch@HelmholtzMunich; München; Vor Ort; EUR 52.000 - 68.000; Vollzeit

Benefits dieser Stelle

International Staff Service

Relocation assistance

State-of-the-art infrastructure

30 days annual leave

Health promotion

Career counseling

Scientific training

Zusammenfassung

Helmholtz Munich invites applications for a research position in the RNA Quality Control lab at Neuherberg near Munich. You will develop iPSC-based models, perform nascent transcriptomics, RNA-seq and proteomics, and interpret results to advance understanding of transcription termination and innate immunity.

You will present findings at meetings and prepare publications. The ideal candidate holds a Master’s degree in biology or related field, has strong molecular biology and cell culture skills,

Qualifikationen

• Master's degree (or equivalent) in biology, biochemistry, molecular medicine or a related field, completed by the start date.
• Solid experimental foundation in molecular biology and mammalian cell culture.
• Good written and spoken English.

Aufgaben

• Investigate how transcription elongation and termination failure generates immunogenic double-stranded RNA.
• Build iPSC-based models using endogenous tagging and degron systems, and differentiate them into neural progenitors and neurons.
• Generate and analyse data: nascent transcriptomics, RNA-seq, proteomics.
• Present at group meetings, institute seminars and international conferences, and write the work up for publication.

Kenntnisse

Molecular biology

Mammalian cell culture

English proficiency

Ausbildung

Master's degree in biology or related field

Tools

CRISPR genome editing

Jobbeschreibung

Illustration: Ella Maru Studio.

102999

Full time

39 hrs./week

Neuherberg near Munich

At Helmholtz Munich, we develop groundbreaking solutions for a healthier society in a rapidly changing world. We believe that diverse perspectives drive innovation. Through strong partnerships, we accelerate the transfer of new ideas from the lab to real-life applications, improving lives. The Helmholtz Pioneer Campus (HPC) founded and funded by Helmholtz Munich stands for the intelligent fusion of biomedical sciences, engineering and digitization. At HPC, top scientific talents from across the globe work together on the development of novel solutions that make a difference in the prevention, diagnosis and treatment of diseases.

The RNA Quality Control lab studies what happens when RNA polymerase II fails to reach the end of a gene, and how the double-stranded RNA (dsRNA) released by that failure drives disease.

We work with a set of methods, we are expert in covering nascent transcriptomics, degron-based protein depletion, iPSC-derived neural models and we will train you in them.

In return, we anticipate that you deploy them well and understand what each experiment tests, and think critically about the results.

Your input will help shape our future research direction.

Your tasks

• Investigate how transcription elongation and termination failure generates immunogenic double-stranded RNA
• Build iPSC-based models using endogenous tagging and degron systems, and differentiate them into neural progenitors and neurons
• Generate and analyse your own data: nascent transcriptomics, RNA-seq, proteomics
• Design and interpret your own experiments
• Present at group meetings, institute seminars and international conferences, and write your work up for publication

Your profile

• Master's degree (or equivalent) in biology, biochemistry, molecular medicine or a related field, completed by the start date
• Solid experimental foundation in molecular biology and mammalian cell culture
• You are feedback driven: you can sense one clear guidance cue to change how you work
• Motivated to work at the bench and at the terminal; prior coding experience is not required, we will teach it
• Good written and spoken English

Desirable Qualifications

• Experience with iPSC culture, neural differentiation or CRISPR genome editing
• Experience with next-generation sequencing or its analysis
• Familiarity with R or Unix
• Background in transcription, RNA biology, innate immunity or the stress response
• Interest in helping set the direction of a group in its first year

This position provides the opportunity for you to build specialist knowledge whilst developing key professional experience, both of which will help you to make strides in your scientific career.

Remuneration and social security benefits are based on the German Collective Wage Agreement for Public-Sector Employees at Federal Level (EG 13 50% TV EntgO Bund). In addition, there is also the possibility of receiving an allowance if the applicable conditions are met. The position has an (initial) fixed term of three years but may be extended under certain circumstances.

Above: healthy cells. Below: cells where transcription termination has failed, with double-stranded RNA (yellow) building up in the cytoplasm.

You will make these cells, treat them, and analyse the sequencing data that explains why they fail. No prior coding experience needed - we teach that part.

Benefits

International Staff Service

Support with the relocation and integration process in Germany

Scientific Resources

State-of-the-art infrastructure and Core Facilities

Recreation

30 days annual leave, flexi days, plus public holidays

Health Promotion

Sports, company doctor, mental health initiatives

Career counseling

Career Center for PhD candidates

Scientific Training

Helmholtz Munich Graduate School

• Career Development: Postdoc program, scientific training & career center with tailored offers
• Recreation: 30 days annual leave, flexi days, plus public holidays
• International Staff Service: Support with the relocation and integration process in Germany
• Health Promotion: Sports, company doctor, mental health initiatives

If you have any questions, feel free to contact Dr. Apoorva Baluapuri, [e-mail], who will be happy to help.

Our recruiting is decentralized - your application will be reviewed directly by the specialist department in which you could work in the future.

We are committed to promoting a culture of diversity and welcome applications from talented people regardless of gender, cultural background, nationality, ethnicity, sexual identity, physical abilities, religion or age. Qualified applicants with physical disabilities will be given preference.

Learn more about diversity at Helmholtz Munich

Our commitment

Helmholtz Munich

Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)

Helmholtz Pioneer Campus

Ingolstädter Landstraße 1

85764 Neuherberg

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