PhD student researcher

GIZ-Nord; Göttingen; Vor Ort; EUR 16.000 - 23.000; Teilzeit

Zusammenfassung

The Institute of Cardiovascular Physiology at the University Medical Center Göttingen invites applications for a PhD student researcher to work on compartment-specific mitochondrial redox signalling and protein translation.

The project uses DAO- and SuperNova-based ROS tools in HEK293 cells, human iPSC-derived cardiomyocytes and neurons, with live-cell imaging, redox proteomics, translation assays and multi-omics integration.

Qualifikationen

• Completed Master of Sciences or equivalent in a Life Sciences subject (molecular medicine, biology, chemistry, physics, …)
• Experience in live-cell fluorescence imaging and quantitative image analysis
• Experience with genetically encoded biosensors, redox biology, mitochondrial biology or protein translation
• Practical experience with mammalian cell culture; experience with iPSC-derived cardiomyocytes or neurons is an advantage
• Experience with molecular cloning, viral transduction, CRISPR-based approaches or engineered cell model
• Interest in integrating imaging data with proteomics, transcriptomics or functional assays
• Strong organisational skills and the ability to work independently in an interdisciplinary research environment
• Excellent communication skills and fluency in English

Aufgaben

• Establish, maintain and validate compartment-targeted ROS-generating cell models
• Perform quantitative live-cell imaging using genetically encoded redox biosensors
• Analyse compartment-specific ROS propagation and redox signalling
• Contribute to redox proteomics, translation assays and mitochondrial functional studies
• Work with iPSC-derived cardiomyocytes and neuronal models
• Integrate imaging, proteomic, transcriptomic and functional datasets
• Coordinate experimental workflows with collaboration partners
• Contribute to publications, presentations and supervision of junior researchers

Kenntnisse

Live-cell imaging

Quantitative image analysis

Genetically encoded biosensors

Redox biology

Mitochondrial biology

Protein translation

English fluency

Independent interdisciplinary work

Ausbildung

Master of Sciences in Life Sciences

Tools

Mammalian cell culture

CRISPR-based approaches

Viral transduction

iPSC-derived cardiomyocytes or neurons

Molecular cloning

Jobbeschreibung

The Institut für Herz- und Kreislaufphysiologie of the University Medical Center Göttingen is looking for a new position for the earliest start date

PhD student researcher (f/m/d)

part time , 25,025 hours per week, 3 Years

The Institute of Cardiovascular Physiology at the University Medical Center Göttingen invites applications for a PhD student researcher position to work within a project on compartment-specific mitochondrial redox signalling and protein translation. The project investigates how localized production of H₂O₂ and O₂•⁻ within mitochondrial subcompartments and the cytosol regulates thiol-dependent redox networks, mitochondrial and cytoplasmic protein translation, and cellular adaptation. The work will use genetically encoded ROS-generating systems, including DAO- and SuperNova-based tools, in HEK293 cells, human iPSC-derived cardiomyocytes and neurons. The project combines live-cell redox imaging, engineered cell models, redox proteomics, translation assays, mitochondrial functional analyses and multi-omics integration.

• establish, maintain and validate compartment-targeted ROS-generating cell models
• perform quantitative live-cell imaging using genetically encoded redox biosensors
• analyse compartment-specific ROS propagation and redox signalling
• contribute to redox proteomics, translation assays and mitochondrial functional studies
• work with iPSC-derived cardiomyocytes and neuronal models
• integrate imaging, proteomic, transcriptomic and functional datasets
• coordinate experimental workflows with collaboration partners
• contribute to publications, presentations and supervision of junior researchers

Completed Master of Sciences or equivalent in a Life Sciences subject (molecular medicine, biology, chemistry, physics, …)

• experience in live-cell fluorescence imaging and quantitative image analysis
• experience with genetically encoded biosensors, redox biology, mitochondrial biology or protein translation
• practical experience with mammalian cell culture; experience with iPSC-derived cardiomyocytes or neurons is an advantage
• experience with molecular cloning, viral transduction, CRISPR-based approaches or engineered cell model
• interest in integrating imaging data with proteomics, transcriptomics or functional assays
• strong organisational skills and the ability to work independently in an interdisciplinary research environment
• excellent communication skills and fluency in English

We offer

• Structured PhD training within GAUSS/GGNB with additional and optional training in the the RTG2824 “Heart and brain diseases: integrative research across organs”
• a scientifically ambitious and well-supported project within a highly collaborative environment
• access to established DAO- and SuperNova-based ROS tools, redox biosensors and iPSC-derived cell models
• close collaboration with experts in redox proteomics, mitochondrial translation, iPSC engineering, mitochondrial imaging, metabolic flux analysis and mitochondrial quality control
• an excellent scientific environment at the University Medical Center Göttingen
• opportunities for publications, conference participation, methodological training and career development

We look forward to receiving your application!

The University Medical Center Göttingen (UMG) unites the Medical Faculty of the Georg-August University and the University Hospital in an integration model. With around 9,700 employees, the UMG and its subsidiaries are one of the largest employers in the region. Our clinics and institutes stand for high-quality patient care, excellent research, modern teaching and transfer of innovative technology.

Göttingen as a city of science is located in the center of Germany and the University Medical Center Göttingen is integrated into an attractive network of university and non-university scientific institutions.

Our goal as University Medical Center Göttingen is professional equality for all genders. We strive to equalize the gender ratio in areas where there is underrepresentation. The University Medical Center Göttingen is particularly committed to the professional participation of severely disabled people and therefore welcomes applications from severely disabled people. In the case of equal suitability, applications from severely disabled persons will be given preferential consideration in accordance with the relevant regulations. We kindly ask you to indicate a disability/equal status in your letter of application in order to safeguard your interests.