Contractor: Discovery Cellular Assay Development

Monte Rosa Therapeutics, Inc.; Basel; On-site; CHF 90,000 - 130,000; Part time

Job summary

Monte Rosa Therapeutics, Inc. is seeking a hands-on Contractor – Discovery Cellular Assays to advance our hit-to-lead efforts. You will design and execute cell-based assays, engineer mammalian cell lines, and apply molecular biology techniques to support compound profiling and mechanism-of-action studies.

Collaboration with cross-functional teams and external partners/CROs is expected, with a focus on developing robust workflows and scalable assays suitable for screening.

Qualifications

• Bachelor's degree in Biochemistry, Biotechnology, Cell Biology, Molecular Biology, or related Life Sciences discipline.
• 2–3+ years of industry or research experience in cellular and molecular biology.
• Strong hands-on experience with mammalian cell culture and cell-line generation/engineering is essential.
• Practical experience with CRISPR/Cas9 gene editing and characterization of engineered cell lines is highly desirable.
• Experience with cell-based assay development and execution, ideally in a drug discovery or compound-profiling environment.
• Experience with protein extraction and Western blotting.
• Familiarity with assay technologies such as fluorescence, luminescence, TR-FRET, AlphaScreen, or related homogeneous assay platforms.
• Experience with high-throughput screening, automation, or liquid-handling platforms is a plus.

Responsibilities

• Design, develop, optimize, and execute cell-based assays to support compound profiling and mechanism-of-action studies
• Perform a broad range of molecular and cellular biology techniques, including:
• Mammalian cell culture and cell-line maintenance
• CRISPR/Cas9-mediated gene editing, including knock-in/knock-out approaches
• Generation, isolation, and characterization of engineered cell lines
• DNA/RNA isolation and analysis
• Protein extraction, quantification, and Western blotting
• Functional cellular readouts such as proliferation, viability, reporter assays, and cytokine measurements
• Implement and execute assays using a range of detection technologies, including fluorescence, luminescence, TR-FRET, AlphaScreen, or related platforms
• Develop and optimize assay protocols, including appropriate controls, assay conditions, and performance parameters, and troubleshoot experimental challenges independently
• Support the transition of assays from development into reproducible and scalable workflows suitable for compound profiling and screening
• Utilize automated liquid-handling systems and laboratory robotics where appropriate
• Analyze and interpret experimental data, including dose-response and other quantitative assay data, and communicate results and conclusions clearly to project teams
• Maintain accurate experimental records, protocols, and data in accordance with company procedures and good laboratory practices
• Prepare cell banks, reagents, and stock solutions and ensure the availability and quality of key experimental materials
• Collaborate effectively with internal cross-functional teams and external partners/CROs to execute experimental plans and resolve technical issues
• Contribute to continuous improvement of laboratory processes, assay platforms, and experimental workflows
• Ensure compliance with laboratory safety procedures and applicable company guidelines

Skills

Mammalian cell culture

Cell-line engineering

CRISPR/Cas9

Molecular biology

Assay development

Protein analysis (Western blot)

Education

Bachelor's degree in Biochemistry/Biology/Life Sciences

Tools

Automated liquid-handling systems

Job description

Monte Rosa Therapeutics, Inc. is seeking a hands-on Contractor – Discovery Cellular Assays to advance our hit-to-lead efforts. You will design and execute cell-based assays, engineer mammalian cell lines, and apply molecular biology techniques to support compound profiling and mechanism-of-action studies.

Collaboration with cross-functional teams and external partners/CROs is expected, with a focus on developing robust workflows and scalable assays suitable for screening.