Associate Research Grade Production

etherna immunotherapies; Niel; On-site; EUR 45,000 - 65,000; Full time

Job summary

etherna immunotherapies seeks an Associate Research Grade Production to lead the manufacture of research grade mRNA-LNPs, including lipid stock preparation, mRNA encapsulation, buffer exchange and sterile filtration.

You will perform in-process and release characterization (particle size, zeta potential, encapsulation, pH/osmolality) and prepare, store, and monitor LNP batches. Collaboration with mRNA production, QC and Process Development is essential.

Responsibilities

• Produce research grade mRNA-LNPs, including lipid stock preparation, mRNA encapsulation (e.g., microfluidic mixing), buffer exchange/purification, concentration and sterile filtration.
• Perform in-process and release characterization of LNP formulations (e.g., particle size and polydispersity by DLS, zeta potential, encapsulation efficiency and mRNA content by RiboGreen assay, pH and osmolality).
• Prepare, aliquot and store LNP batches under appropriate conditions and follow up on their stability.
• Execute new techniques/assays and support the optimization and scale-up of formulation processes. Work collaboratively with other departments (mRNA production, QC, Process Development) and/or project managers.
• Maintain appropriate files related to ongoing formulations. Write formulation protocols, batch records, analytical test methods and reports if necessary.
• Keep track of QC results of ongoing productions.

Job description

The Associate Research Grade Production’s duties shall include, but not be limited to:

• Primary responsibility will be to produce research grade mRNA-LNPs, including lipid stock preparation, mRNA encapsulation (e.g., microfluidic mixing), buffer exchange/purification, concentration and sterile filtration.
• Perform in-process and release characterization of LNP formulations (e.g., particle size and polydispersity by DLS, zeta potential, encapsulation efficiency and mRNA content by RiboGreen assay, pH and osmolality).
• Prepare, aliquot and store LNP batches under appropriate conditions and follow up on their stability.
• Execute new techniques/assays and support the optimization and scale-up of formulation processes.Work collaboratively with other departments (mRNA production, QC, Process Development) and/or project managers.
• Maintain appropriate files related to ongoing formulations. Write formulation protocols, batch records, analytical test methods and reports if necessary.
• Keep track of QC results of ongoing productions.