Research Technician – Bernecky group

Institute of Science and Technology Austria; Wien; Vor Ort; EUR 37.800 - 46.200; Vollzeit

Zusammenfassung

A leading research institute in Vienna seeks a self-motivated technician to support research on chromatin-binding protein complexes. Responsibilities include molecular cloning, protein expression and purification, and optimization of assays. The ideal candidate has a Masters or PhD in biochemistry, practical knowledge of molecular biology, and experience with ÄKTA chromatography systems. The position offers a competitive salary and opportunities for personal growth in a dynamic research environment.

Qualifikationen

• Strong theoretical and practical knowledge of molecular biology and protein purification.
• Experience in designing purification strategies and biochemical assays.
• Ability to perform experiments with high reproducibility.

Aufgaben

• Provide research support investigating chromatin-binding multisubunit protein complex.
• Design and prepare plasmids for molecular cloning.
• Optimize and perform biochemical and biophysical assays.

Kenntnisse

Molecular cloning

Protein expression

Protein purification

Biochemical assays

Ausbildung

Masters or PhD degree in biochemistry or related subject

Tools

ÄKTA chromatography systems

Jobbeschreibung

• € 42,000* gross/year – Position limited until August 2027

Your Responsibilities

The Bernecky group aims to uncover critical mechanisms of RNA regulation during and after transcription. We use purification, reconstitution, and biochemical and structural analysis (particularly cryo-EM) to characterize protein-nucleic acid complexes and investigate their function. We are looking for a self‑motivated, efficient, and reliable technician to provide research support for a project in which we investigate the activity and structure of a chromatin‑binding multisubunit protein complex. This will include the following tasks:

• Molecular cloning (design and preparation of plasmids)
• Protein expression in insect and mammalian cells
• Protein purification, quantification, and quality control
• Optimization and performance of biochemical and biophysical assays

Your Profile

• Masters or PhD degree in biochemistry or a related subject
• Theoretical and practical knowledge of molecular biology and protein purification, including design of purification strategies and biochemical assays
• Experience using ÄKTA chromatography systems for multi‑step protein purification
• Ability to perform experiments with high reproducibility and efficiently organize your time
• Applications should include a short letter of motivation, a CV, and contact information for 2 referees

ISTA is growing. Grow with us!

Institute of Science and Technology Austria (ISTA) is a constantly growing international institute for conducting world‑class research in mathematics, computer science, life sciences & physical sciences. We strive to recruit passionate professionals from across the world over all fields who strive to support our goal of excellent research. Located within a beautiful campus on the outskirts of Vienna, we offer multiple opportunities for personal growth in a stable working environment. Get an insight!

*This position comes with possible overpayment depending on education, qualification and work experience. For more information on how ISTA processes your personal data, please refer to www.ista.ac.at/data-protection.