Yeast Display Scientist II – Protein Design & ML Data

Profluent Bio; Emeryville (CA); Presencial; USD 155.000 - 160.000; Jornada completa

Ventajas ofrecidas por este puesto de trabajo

Equity participation

401(k) with strong match

Health/dental/vision insurance

PTO (paid time off)

Professional development

Descripción de la vacante

Profluent Bio in Emeryville, CA seeks a Scientist II to own and advance the yeast surface display platform for binder generation. You will build workflows from library construction through FACS and NGS readouts, and partner with ML and Protein Design teams to define model-ready data.

The role increasingly guides platform direction, with opportunities to scale across targets and libraries, contribute to developability considerations, and mentor others in a fast-paced startup environment.

Formación

• PhD in Molecular Biology, Biochemistry, Protein Engineering, Biophysics, Immunology, or related field with 3+ years industry experience.
• Deep hands-on experience with yeast surface display, including library construction, induction and expression optimization, and multi-round selection campaigns.
• Proven track record of generating and screening libraries for binder discovery or optimization.
• Strong flow cytometry and FACS expertise, including gating strategy and data interpretation.
• Working knowledge of NGS-based screening workflows and turning data into quantitative readouts.
• Strong molecular biology skills (cloning, mutagenesis, DNA assembly).
• Ability to define and uphold assay quality standards.
• Solid understanding of protein engineering and developability considerations.

Responsabilidades

• Establish and validate a yeast surface display platform for binder generation, including library design and FACS-based selection.
• Design and execute yeast display campaigns across binder formats against various targets.
• Develop robust, repeatable workflows with controls and QC; characterize reproducibility and binding signals.
• Integrate NGS readouts into selections and interpret deep mutational scanning data.
• Collaborate with ML and Protein Design teams to define campaign targets and data requirements.
• Work with Bioinformatics to ensure dataset traceability to campaigns.

Conocimientos

Yeast display

Flow cytometry

NGS workflows

Cloning & mutagenesis

Protein engineering

Model collaboration

Assay development

Educación

PhD in Molecular Biology

Herramientas

FACS instruments

NGS platforms

Cloning tools

Bioinformatics pipelines

Descripción del empleo

Profluent Bio in Emeryville, CA seeks a Scientist II to own and advance the yeast surface display platform for binder generation. You will build workflows from library construction through FACS and NGS readouts, and partner with ML and Protein Design teams to define model-ready data.

The role increasingly guides platform direction, with opportunities to scale across targets and libraries, contribute to developability considerations, and mentor others in a fast-paced startup environment.