Sr. Scientist/Principal Scientist - Bioanalysis

Citytx; Cambridge (MA); On-site; USD 88,000 - 122,000; Full time

Job summary

A leading company is seeking a highly motivated Sr. or Principal Scientist specializing in bioanalysis to join their research team in Cambridge. The ideal candidate will have expertise in LC/MS-based oligonucleotide analysis, playing a critical role in method development and data interpretation. This full-time opportunity is onsite and geared towards candidates with advanced degrees and substantial industry experience.

Qualifications

• 5+ years of relevant industry experience for Ph.D. or 8+ years for M.S.
• Deep expertise in LC-MS/MS and HRMS techniques.
• Experience with ASO, siRNA, or mRNA platforms.

Responsibilities

• Develop and optimize LC/MS methods for oligonucleotide analysis.
• Design in vitro hepatocyte studies for metabolic evaluations.
• Collaborate with cross-functional teams to support project objectives.

Skills

mass spectrometry

analytical chemistry

biomolecule characterization

metabolite identification

data interpretation

Education

Ph.D. in Analytical Chemistry or related field

M.S. in Analytical Chemistry or related field

Tools

Xcalibur

MassLynx

Compound Discoverer

Job description

Sr. Scientist/Principal Scientist - Bioanalysis

Sr. Scientist/Principal Scientist - Bioanalysis

We are seeking a highly motivated and skilled Sr. or Principal Scientist with expertise in LC/MS-based analysis of oligonucleotides to join our growing research team. The ideal candidate will have hands-on experience with mass spectrometry-based structural elucidation, including metabolite identification (Met ID) and preferably, in vitro hepatocyte cell culture systems. This individual will play a key role in supporting both discovery and development-stage programs through advanced analytical method development, sample analysis, and data interpretation. Please note this is an onsite opportunity.

Responsibilities

• Develop, optimize, and qualify LC/MS methods for qualitative and quantitative analysis of oligonucleotide-based therapeutics.
• Design and execute in vitro hepatocyte incubation studies to evaluate metabolic stability and biotransformation pathways.
• Utilize high-resolution mass spectrometry (HRMS) for sequence verification, metabolite profiling and protein biomarker analysis.
• Perform structural characterization and metabolite identification (Met ID) of oligonucleotides and related compounds.
• Collaborate closely with cross-functional teams in medicinal chemistry, DMPK, and biology to support project objectives.
• Analyze, interpret, and present experimental data clearly and concisely to key stakeholders.
• Ensure all work complies with regulatory guidelines, standard operating procedures (SOPs), and data integrity principles.
• Prepare Bioanalysis and ADME reports for IND/CTA filing.
  
  

Qualifications

• Ph.D. in Analytical Chemistry, Biochemistry, Pharmaceutical Sciences, or a related field with 5+ years of relevant industry experience; or M.S. with 8+ years of industry experience.
• Deep expertise in LC-MS/MS and HRMS techniques for biomolecule characterization, especially oligonucleotides.
• Experience in antisense oligonucleotide (ASO), siRNA, or mRNA therapeutic platforms.
• Proficiency with mass spectrometry software (e.g., Xcalibur, MassLynx, Compound Discoverer, Analyst, etc.).
• Demonstrated ability to troubleshoot complex analytical challenges and drive results.
• Excellent organizational, communication, and documentation skills.
  
  

Preferred Qualifications

• Proven experience in oligonucleotide metabolism, Met ID, and in vitro ADME studies using hepatocytes or similar systems.
• Experience with LC/MS-based peptide and protein quantification
• Knowledge of regulatory bioanalytical guidelines (FDA, EMA) is a plus.
  
  

City Therapeutics is accepting resumes/CVs from direct applicants only. Recruitment Agency submissions will not be accepted at this time.

City Therapeutics is an Equal Opportunity and Affirmative Action employer committed to a culturally diverse workforce. All qualified applicants will receive consideration for employment without regard to race; color; creed; religion; national origin; age; ancestry; nationality; marital, domestic partnership or civil union status; sex, gender, gender identity or expression; affectional or sexual orientation; disability; veteran or military status or liability for military status; domestic violence victim status; atypical cellular or blood trait; genetic information (including the refusal to submit to genetic testing) or any other characteristic protected by law.

Seniority level

• Seniority level

  Mid-Senior level

Employment type

• Employment type

  Full-time

Job function

• Job function

  Research, Analyst, and Information Technology

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