Senior Research Associate, Single-Cell RNA Sequencing

Real Staffing Group; Boston (MA); On-site; USD 52,000 - 74,000; Full time

Benefits offered by this job

Medical benefits

Dental benefits

Vision benefits

Job summary

Pharmaceutical Company is seeking a Senior Research Associate with expertise in single-cell RNA sequencing to join an analytical development team on a one-year, full-time, onsite contract in Boston, MA. The role focuses on high-quality single-cell sample processing and library preparation for clinical research programs.

You will independently plan, execute, and troubleshoot experiments using platforms such as 10x Genomics Chromium, perform tissue dissociation and cell prep, and prepare cDNA

Qualifications

• Bachelor's degree in molecular biology, cell biology, genomics, biotechnology, or related.
• Master's degree with 2+ years of relevant experience is acceptable.

Responsibilities

• Plan, execute, and troubleshoot single-cell RNA-sequencing experiments.
• Prepare libraries for gene expression and multiomic applications.
• Coordinate sequencing submissions and maintain sample metadata.
• Mentor junior team members and assist in protocol optimization.

Skills

Single-cell RNA sequencing

NGS library prep

Cell preparation

cDNA amplification

Library construction

Education

Bachelor's degree in molecular biology

Master's degree + experience

Tools

10x Genomics Chromium

Bioanalyzer

TapeStation

Job description

Company: Pharmaceutical Company

Role: Senior RA, Single-Cell RNA Sequencing

Contract: 1 year W2 contract

Location: onsite in Boston

Pay rate-: 46/hour

Start date: ASAP

Benefits: medical, dental and vision

We are seeking a Senior Research Associate with expertise in single-cell RNA sequencing to join an analytical development molecular biology team for a one-year contract. This full-time, onsite role in Boston, MA focuses on supporting clinical research programs through high-quality single-cell sample processing and library preparation.

The successful candidate will independently plan, execute, and troubleshoot single-cell RNA-sequencing experiments using established and emerging genomics platforms. Responsibilities include cell and tissue dissociation, viability assessment, enrichment, sorting coordination, cryopreservation, and preparation of single-cell suspensions from a variety of sample types. You will perform partitioning, barcoding, cDNA generation, amplification, and library preparation, utilizing workflows such as 10x Genomics Chromium. Additional duties involve preparing libraries for gene expression and multiomic applications, conducting quality-control assessments with instruments like Bioanalyzer and TapeStation, and coordinating sequencing submissions while maintaining accurate sample metadata.

You will collaborate with scientists and project teams to design experiments, advise on technical variables, identify risks, and recommend mitigations for challenging samples. The role includes leading investigations into assay failures, optimizing protocols and instruments, developing standardized procedures, and mentoring junior team members.

Candidates should hold a bachelor's degree in molecular biology, cell biology, genomics, biotechnology, or a related discipline with at least 5 years of relevant experience, or a master's degree with 2 years of experience. Hands‑on experience with single‑cell RNA‑sequencing workflows and platforms such as 10x Genomics Chromium is required, along with strong knowledge of RNA handling and molecular biology techniques. Excellent organizational skills, attention to detail, and the ability to manage multiple experiments in a fast-paced environment are essential.

Experience with multiomic single-cell assays, laboratory automation, and regulated documentation practices is preferred. The position requires regular laboratory work with biological specimens and may occasionally require schedule flexibility.

Must-Have Skills: single-cell RNA sequencing, next-generation sequencing library preparation, 10x Genomics Chromium, cell preparation, cDNA amplification, library construction, Bioanalyzer

Nice-to-Have Skills: multiomic single-cell assays, single-cell ATAC-seq, CITE-seq, immune profiling, cell hashing, feature barcoding, spatial transcriptomics, laboratory automation, liquid-handling platforms, GxP, regulated documentation, biomarker research, translational research, organizational, attention to detail, troubleshooting

EOE Statement: Specialist Staffing Group is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or veteran status.

In addition to base pay, employees may be eligible for benefits such as health insurance, health savings accounts, retirement savings plans, life and disability insurance, and paid leave where required by applicable law. Eligibility may vary based on factors such as location and hire date and is subject to change.