Scientist/Sr. Scientist, Protein Purification

Zealand Pharma; Cambridge (MA); Hybrid; USD 125,000 - 170,000; Full time

Job summary

Zealand Pharma in Cambridge, MA is seeking a Scientist or Senior Scientist in Protein Purification and Analysis to deliver high-purity protein and bioconjugate samples for downstream mass spectrometry and structural biology studies. You will purify recombinant proteins and their bioconjugates, leveraging SEC, IEX, affinity, RP-HPLC, and CE at analytical and preparative scales.

You will work with analytical characterization methods (MS, biophysical assays, flow cytometry) and help develop

Qualifications

• PhD preferred; postdoc experience considered.
• 2+ years post-degree experience for Scientist; 4+ years for Senior Scientist.
• Hands-on purification using SEC, IEX, affinity, RP-HPLC, CE.
• Experience with ADCs/AOCs or bioconjugates preferred.

Responsibilities

• Purify recombinant proteins, peptides, and bioconjugates at analytical and preparative scales.
• Support purification workflows and analytical characterization.
• Collaborate across cross-functional teams in a new research center.
• Develop automated, high-throughput purification approaches.

Skills

Protein purification

AKTA systems

SEC

IEX

Affinity chromatography

RP-HPLC

CE-based methods

Mass spectrometry

Biophysical analysis

Flow cytometry

Education

PhD in Analytical Chemistry

Protein Engineering

Biochemistry

BS/MS with industry experience

Tools

AKTA pure

AKTA avant

Job description

## Scientist/Sr. Scientist, Protein PurificationApplylocations: Cambridge, MA, UStime type: Full timeposted on: Posted Yesterdayjob requisition id: JR362Does it excite you to be the go-to purification expert delivering high-purity samples for cutting-edge structural biology and mass spec studies? If this sounds like you, and you are ready to bring deep purification expertise to a new discovery organization, then you could be our new colleague.**Your new position** As a Scientist or Senior Scientist in Protein Purification and Analysis, you will play an important scientific role supporting discovery programs, with a primary emphasis on high-quality purification of protein-based therapeutics and their bioconjugates. You will work hands-on with a broad range of modern protein purification technologies, with complementary analytical characterization, at the inception of our new Cambridge, MA research center. This role suits a scientist with deep, hands-on expertise in protein and bioconjugate purification, scientific curiosity, and a desire to grow in scope and responsibility.**We offer exciting responsibilities:*** Serve as a primary supplier of high-purity protein and bioconjugate samples formatted to meet the requirements of downstream mass spectrometry (e.g., native MS, HDX-MS) and structural biology methods (e.g., cryo-EM, X-ray crystallography)* Execute and support purification of recombinant proteins, peptides, and their bioconjugates (e.g., ADCs, AOCs) to support discovery programs from early research through lead-series selection* Apply a broad range of purification techniques, including SEC, IEX, affinity, RP-HPLC, and CE-based methods, at analytical and preparative scales* Support purification and characterization of challenging targets and formats, such as membrane proteins (e.g., GPCRs), in vitro-reconstituted target complexes, and peptide-Fc or lipidated conjugates* Support analytical characterization to confirm identity, purity, concentration, and stability using mass spectrometry, chromatographic, biophysical, and flow cytometry-based methods* Contribute to the development and optimization of automated and high-throughput purification workflows, improving speed, reproducibility, and data quality**Your profile** You bring deep, hands-on purification expertise and scientific curiosity, with a strong conceptual understanding of protein biophysics and stability principles. You thrive in a collaborative, multidisciplinary environment and are motivated to help shape purification workflows and a new research center from an early stage. You communicate scientific data, methods, and assumptions clearly and work effectively across cross-functional project teams.* PhD in Analytical Chemistry, Protein Engineering, Biochemistry, or a related field preferred (postdoctoral training preferred); candidates with a BS/MS and significantly more relevant industry experience will also be considered* 2+ years of relevant post-degree experience in protein purification (Scientist); 4+ years (Senior Scientist)* Deep, hands-on expertise in protein and peptide purification, including SEC, IEX, affinity, RP-HPLC, and CE-based methods, at analytical and preparative scales* Experience with complex molecular formats such as ADCs, AOCs, or other bioconjugates strongly preferred* Hands-on experience with AKTA chromatography systems (e.g., AKTA pure, AKTA avant) is desired* Working knowledge of analytical characterization techniques, including mass spectrometry, biophysical methods (e.g., DLS, MALS, SPR, BLI, DSF), and flow cytometry, sufficient to collaborate effectively with analytical specialists**Your new team** You will join Molecular Platform Development, a new organization created to strengthen and advance diverse drug modality capabilities in metabolic disease, based at our new Cambridge, MA research center. The site is a highly collaborative, multidisciplinary discovery hub integrating computational research, chemistry, and biology, and is deeply connected to the Greater Boston life sciences ecosystem.**Let's bond and be bold** We have a unique culture, characterized by excellent teamwork and strong engagement across the organization. And we want you to help us strengthen this culture.Making bonds is at the core of everything we do. From discovering and developing the best new peptide drugs for patients, to engaging with our partners or connecting with each other. We bond through curiosity and playfulness as a team, allowing ourselves to challenge common thinking and drive future innovation. Join us and let's bond and be bold.Salary Range: $125,000 - $170,000Interested in bonding with us? The applications will be reviewed as they come in. While a cover letter is not required, please include a few sentences in your CV explaining your motivation for applying.