Scientist/Sr. Scientist, Protein Production and Purification

Kodiak Sciences; Palo Alto (CA); On-site; USD 110,000 - 160,000; Full time

Job summary

A leading biopharmaceutical company is seeking a Protein Production Scientist to join their dynamic Protein Engineering team. This role offers an exciting opportunity to contribute to innovative therapies addressing high-prevalence ophthalmic diseases by performing protein production and purification in a cutting-edge environment. Candidates with hands-on experience in transient transfection and expertise in protein purification are encouraged to apply for this mid-senior level position.

Qualifications

• 5+ years of relevant industry experience or Ph.D. with 5 years experience for the role.
• Hands-on expertise in protein purification workflows required.
• Commitment to exploring novel purification methods.

Responsibilities

• Perform transient transfection and protein expression in mammalian and bacterial systems.
• Develop and execute purification protocols for antibodies and proteins.
• Analyze purified proteins using SDS-PAGE, DLS, and other techniques.

Skills

Transient transfection in mammalian systems

Protein purification

Attention to detail

Communication skills

Organizational skills

Analytical techniques (SDS-PAGE, UV-Vis)

Education

B.S. or M.S. in Cell Biology, Biochemistry, or related disciplines

Ph.D. in related fields

Tools

AKTA FPLC

KingFisher instrumentation

Job description

Scientist/Sr. Scientist, Protein Production and Purification

Scientist/Sr. Scientist, Protein Production and Purification

Get AI-powered advice on this job and more exclusive features.

Kodiak Sciences Inc. is a fast-growing, clinical-stage biopharmaceutical company focused on transforming ophthalmology through innovative therapeutics. Our Antibody Biopolymer Conjugate Drug (ABCD) platform combines protein engineering, polymer chemistry, small molecule drug discovery, bioconjugation, and drug delivery to address high-prevalence ophthalmic diseases.

We are seeking a motivated and independent Protein Production Scientist to join our Discovery Medicine group as part of the Protein Engineering team. The team is responsible for designing, engineering, and characterizing antibodies and novel drug formats for retinal disease treatment. The successful candidate will play a key role in driving research efforts through protein production and purification. This is an exciting opportunity to contribute to cutting-edge therapeutics development in a dynamic, interdisciplinary environment.

Responsibilities:

• Perform transient transfection and protein expression in mammalian (e.g., HEK293, CHO) and bacterial systems.
• Develop and execute purification protocols for antibodies and other protein formats.
• Purify protein-polymer and protein-drug conjugates using established and novel methods.
• Analyze purified proteins using techniques such as SDS-PAGE, DLS, mass photometry, and isoelectric focusing (IEF).
• Maintain and troubleshoot mammalian cell culture systems.
• Coordinate resources to schedule protein production and purification.
• Document protein production processes, prepare reports, and communicate results effectively with the team.
• Maintain accurate and detailed notebook entries and protein inventory records.
• Perform general laboratory tasks independently to support project goals.

Qualifications:

Education

• B.S. or M.S. in Cell Biology, Biochemistry, Molecular Biology, or related disciplines with 5+ years of relevant industry experience, or Ph.D. in the same fields with 5 or fewer years of relevant experience in an academic or industry setting.

Required Skills

• Hands-on experience with transient transfection in mammalian systems (e.g., HEK293, CHO).
• Strong expertise in protein purification including column chromatography and batch-mode purification.
• Familiarity with AKTA FPLC and KingFisher instrumentation.
• Commitment to protein production and drive to explore novel, high-throughput purification methods.
• Proficiency in protein analytical techniques such as SDS-PAGE and UV-Vis (e.g., Nanodrop).
• Exceptional attention to detail and commitment to accurate documentation.
• Strong communication, organizational, and interpersonal skills.

Preferred Skills

• Hands-on expertise in antibody expression and purification workflows.
• Experience with antibody-drug conjugates (ADCs) or protein-polymer conjugates.

Seniority level

• Seniority level

  Mid-Senior level

Employment type

• Employment type

  Full-time

Job function

• Job function

  Research, Analyst, and Information Technology

Referrals increase your chances of interviewing at Kodiak Sciences by 2x

Sign in to set job alerts for “Senior Scientist” roles.

Scientist I-III/Senior/Lead - Polymer Formulation & Materials Characterization

Alameda, CA $110,000.00-$160,000.00 1 week ago

Menlo Park, CA $100,000.00-$150,000.00 2 weeks ago

San Jose, CA $160,000.00-$220,000.00 1 week ago

Senior Staff Scientist, Computational Chemistry

South San Francisco, CA $170,000.00-$185,000.00 5 days ago

Principal/Senior Scientist, Analytical Science

Senior Scientist/Principal Scientist, Biochemist and Cellular Neurobiologist

South San Francisco, CA $163,000.00-$201,000.00 5 days ago

Senior Scientist II, Analytical Development

Milpitas, CA $115,900.00-$197,000.00 3 weeks ago

South San Francisco, CA $156,000.00-$296,500.00 2 weeks ago

Sr. Staff Scientist, Analytical Development

Principal Scientist / Director, Clinical Pharmacology

Scientist 1/2/Senior Scientist, Analytical Development

Sr. Scientist, Small-Molecule Drug Substance Development and Manufacturing

Hayward, CA $150,000.00-$170,000.00 3 days ago

Senior Scientist - Chemical Development and Drug Substance Manufacturing

Senior Principal Research Scientist, Analytical Chemistry

South San Francisco, CA $156,000.00-$296,500.00 2 weeks ago

San Francisco Bay Area $55.00-$65.00 2 weeks ago

Senior Staff Scientist, Medicinal Chemistry

Milpitas, CA $141,000.00-$227,000.00 1 week ago

Research And Development Scientist, Food Product / Process Developer

South San Francisco, CA $140,000.00-$150,000.00 6 days ago

Senior Manufacturing Development Chemist

Sunnyvale, CA $112,500.00-$168,750.00 1 week ago

Senior Scientist I/II, Biologics Analytical R&D

We’re unlocking community knowledge in a new way. Experts add insights directly into each article, started with the help of AI.