Scientist, Platform Technologies

Tenvie Therapeutics, Inc.; San Francisco (CA); On-site; USD 119,000 - 157,000; Full time

Job summary

A leading biotechnology company is seeking a Scientist in Platform Technologies to lead protein expression designs and manage external workflows. This role focuses on the purification and characterization of proteins, employing advanced techniques such as chromatography and biophysical methods to support drug discovery initiatives.

Qualifications

• 3 years experience in biotech/biopharma, with 1 year on NanoDSF and Biacore.
• Hands-on experience in protein purification from bacterial expression systems.
• Competence in managing workflows and protein characterization methods.

Responsibilities

• Lead design of protein expression constructs and maintain protein inventory.
• Project manage protein chemistry resources across multiple high-profile projects.
• Utilize biophysical characterization methods for structural studies.

Skills

Protein purification

Chromatographic techniques

Biophysical characterization

Education

Master’s degree in biology, biotechnology, or related field

Tools

Akta FPLC

Agilent HPLC

Thermo Fisher HPLC

NanoDSF

Biacore

Job description

Web Ad

Tenvie Therapeutics, Inc.
Scientist, Platform Technologies
South San Francisco, CA

Overview

Lead role in the design of protein expression constructs and maintenance of a database of constructs and protein inventory, including the purification and characterization of proteins (intracellular, extracellular, and multi-pass membrane) for our small molecule discovery program and the design and development of high content imaging cellular assays.


Responsibilities:

Communicate protocols and manage workflows of external FTEs.

Utilize various methods for biophysical characterization (SPR, DSF, DLS, TM-FSEC) of recombinant proteins samples for structural studies including CryoEM and crystallography.

Project manage protein chemistry resources across multiple high-profile projects.

Design and execute purification of native proteins from serum, tissue, and other biofluids.

Lead troubleshooting efforts for challenging protein expression and purification processes.

Analyze purified proteins using a range of protein characterization methods (including HPLC, SEC-MALS, DLS, SDS/PAGE and others).

Establish methods to characterize and QC proteins for small molecule/protein binding.

Qualifications

Must have a master’s degree in biology, biotechnology, related or the equivalent and 3 years of experience in biotech/biopharma, including with: Hands-on experience in protein purification from bacterial expression systems; Chromatographic techniques including the Akta FPLC platform and Agilent and Thermo Fisher HPLC systems; and, Culture and express recombinant proteins in e. coli. Of the 3 years of experience, 1 year of experience setting up and analyzing experiments from NanoDSF and Biacore instruments.

Salary range for the position: $119,000 – 157,000.