Scientist II, Conjugation

Soufflé Therapeutics; Watertown (MA); On-site; USD 120,000 - 160,000; Full time

Job summary

Soufflé Therapeutics in Watertown, MA is seeking a Scientist II in Protein Science – Conjugation to advance bioconjugation methods and new payload modalities, with ownership from day one.

You will lead conjugation work, perform characterization via SEC-HPLC, SDS-PAGE, and mass spectrometry, and collaborate across internal programs and external collaborations to drive new modalities.

Qualifications

• 6+ years of relevant experience in academia and industry.
• Hands-on protein bioconjugation experience, especially antibody-drug conjugates.
• Experience with purification methods including affinity/IEX chromatography and TFF.
• Analytical skills for characterizing proteins and conjugates (SEC-HPLC, IEX-HPLC, RP-HPLC, SDS-PAGE, MS).

Responsibilities

• Prepare novel bioconjugates for in vitro and in vivo studies.
• Lead conjugation work from planning through execution.
• Advance conjugation modalities, payloads, and formats.
• Represent the conjugation team in cross-functional meetings.

Skills

Protein bioconjugation

Antibody-drug conjugates

Chromatography

Mass spectrometry

Analytical techniques

Cross-functional collaboration

Education

PhD in Chemistry, Biochemistry, Chemical Biology, Bioengineering or related field

MS with 6-10 years of industry conjugation experience

BS with 10+ years' experience in conjugation

Tools

Affinity chromatography

Ion exchange chromatography

Tangential flow filtration

Job description

Soufflé Therapeutics is a biotech company focusing on the cell-type specific delivery of genetic medicines. It's team of driven scientists is making a mark in delivery of oligonucleotides to extrahepatic tissues. We thrive in a purpose-building lab and office space in Watertown. Come and join us in our quest!

Position Summary:

Soufflé Therapeutics is growing its conjugation capabilities to enable state of the art conjugation methodologies to new ligand and payload modalities, expanding internal programs, and new collaborations. As a Scientist II in Protein Science – Conjugation, you'll add hands-on bioconjugation expertise to advance new conjugation methods and enable new molecular modalities across internal programs and external collaborations. If you want to help shape how a growing biotech builds its conjugation chemistry and purification capabilities, this role gives you real ownership from day one.

Key Responsibilities

• Leverage knowledge of protein and conjugation chemistry to prepare novel bioconjugates for in vitro and in vivo studies.
• Lead conjugation work supporting internal programs and collaborations, from planning through execution.
• Drive conjugation chemistry research into new conjugation modalities, payloads, and conjugate formats.
• Perform characterization of conjugates using techniques such as SEC-HPLC, SDS-PAGE, and mass spectrometry.
• Represent the conjugation team in prioritization and cross-functional collaboration meetings.

What Success Looks Like

• In 3 months: You've mastered our core conjugation workflows and techniques and understand the background of current projects
• In 6 months: You're independently executing conjugation experiments, and rigorously analyzing and interpreting data
• In 12 months: Your experimental work is making a measurable impact on company objectives – improving our conjugation process or enabling new payloads or workflows – and you know how to get things done across the organization

Qualifications — Required

• 6+ years of relevant experience (academic and industry combined)
• Hands-on experience with protein bioconjugation, particularly antibody-drug conjugates
• Hands-on experience with protein purification methods, such as affinity chromatography, ion exchange chromatography, and tangential flow filtration
• Proficiency in analytical techniques for characterizing proteins and protein conjugates, such as SEC-HPLC, IEX-HPLC, RP-HPLC, SDS-PAGE, and mass spectrometry

Qualifications — Preferred

• Experience optimizing chemical and enzymatic bioconjugation methods, including site‑specific conjugation
• Experience with multi‑gram‑scale bioconjugation and/or purification, and with HPLC method development, maintenance, and troubleshooting

Education

• Ph.D. in Chemistry, Biochemistry, Chemical Biology, Bioengineering, or a related field with 6 years of experience or MS with 6-10 years of industry experience with conjugation OR a BS with 10+ years' experience

At Soufflé Therapeutics, we are intentional about building an equitable culture. How we treat our people is reflective of this focus. This is an onsite position in Watertown, MA (US) with flexibility as needed for collaboration and external engagements.