Scientist II (Cell Therapy / Genome Engineering)

dawarconsulting; South San Francisco (CA); On-site; USD 83,000 - 90,000; Full time

Benefits offered by this job

Medical

Dental

Vision

Paid Sick Leave

401K

Job summary

Our client, a world leader in biotechnology and life sciences, is seeking a Scientist II (Cell Therapy / Genome Engineering) to support cell therapy development and disease-modeling programs. Location: South San Francisco, CA.

This long-term contract role features a competitive pay rate and comprehensive benefits. The successful candidate will engineer mammalian cells using CRISPR and viral systems, optimize gene-editing workflows, and characterize engineered cells with molecular and genomic

Qualifications

• BS or MS with 5 years industry experience or PhD in Molecular Biology, Cell Biology, Genetics, or related field.
• Strong hands-on mammalian cell culture experience.
• Experience with primary T cells and iPSCs (preferred).

Responsibilities

• Engineer mammalian cells (cancer cell lines, iPSCs, primary cells) using lentiviral/piggyBac systems and CRISPR gene editing.
• Optimize transfection and gene-editing workflows.
• Troubleshoot and refine cell engineering protocols.
• Characterize engineered cells using molecular and genomic assays.
• Maintain, monitor, and quality-check cell lines in culture.

Skills

Mammalian cell culture

CRISPR gene editing

Molecular biology

Cell-based assays

Education

BS/MS + 5 years industry experience or PhD in Molecular Biology, Cell Biology, Genetics, or related field

Tools

Benchling

SnapGene

FlowJo

GraphPad Prism

Job description

Our client, a world leader in biotechnology and life sciences, is seeking a "Scientist II (Cell Therapy / Genome Engineering)".

Location: South SanFrancisco, CA

Duration: Long-Term Contract (Possibility Of Further Extension)

Pay Rate: $60-$65/hr on W2

Company Benefits:

• Medical
• Dental
• Vision
• Paid Sick Leave
• 401K

Job Summary

In this role, you will be supporting genome engineering and synthetic biology initiatives. This role contributes to cell therapy development and disease-modeling programs. The successful candidate will support the needs of the Advanced Cell Engineering Hub, particularly in the engineering and profiling of cells for cell therapy development and disease modeling.

Key Responsibilities

• Engineer mammalian cells (cancer cell lines, iPSCs, primary cells) using lentiviral/piggyBac systems and CRISPR gene editing
• Optimize transfection and gene-editing workflows
• Troubleshoot and refine cell engineering protocols
• Characterize engineered cells using molecular and genomic assays
• Maintain, monitor, and quality-check cell lines in culture

Required Qualifications

• BS/MS + 5 years industry experience or PhD in Molecular Biology, Cell Biology, Genetics, or related field
• Strong hands-on mammalian cell culture experience

Preferred Qualifications

• Experience with primary T cells and iPSCs

Skills in:

• DNA/RNA/protein extraction, molecular cloning, PCR/RT-PCR/ddPCR, FACS, CRISPR editing, siRNA/shRNA, SDS-PAGE, immunoblotting, cell-based assays, NGS, viral vector work
• Familiarity with analysis tools such as Benchling, SnapGene, FlowJo, GraphPad Prism