RNA Processing Research Associate – Mammalian Cells

Planet Pharma; South San Francisco (CA); Sur place; USD 26 000 - 39 000; Plein temps

Résumé du poste

Planet Pharma is seeking a Research Associate to study RNA post-transcriptional processing and regulation in human cells. You will perform experiments, maintain accurate records, and collaborate with the project scientist to interpret results and determine next steps.

The ideal candidate is curious, resourceful, and willing to ask questions, investigate unexpected results, and see experiments through to completion. On-site work in South San Francisco is required.

Qualifications

• Bachelor’s degree in molecular biology, cell biology, biochemistry, genetics, or a related field, with at least 1-2 years of laboratory experience.
• Experience with mammalian cell culture.
• Experience with RNA isolation, RNA quality control, RNA sequencing library preparation, and NGS.
• Experience with one or more of the following: oligonucleotide or siRNA perturbation, reporter assays, immunoprecipitation, Western blotting, or RT-qPCR.
• Familiarity with RNA post-transcriptional processing such as splicing, translation or cleavage and polyadenylation.
• Demonstrated ability to troubleshoot experiments, interpret results in the context of a research question, communicate clearly, and work both independently and collaboratively.

Responsabilités

• Maintain, passage, treat, transfect, and preserve mammalian cell lines. Monitor cell health and recognize potential issues with growth, media, or contamination.
• Perform molecular biology experiments, including DNA and RNA purification, plasmid preparation, PCR, electrophoresis, and preparation of solutions and dilutions.
• Conduct experiments, including reporter and minigene assays, RNA isolation, RT-qPCR, and preparation of next generation sequencing libraries. Perform additional RNA or protein assays as project evolves.
• Prepare reagents, maintain supplies and equipment, and keep the laboratory organized and ready for experiments. Follow applicable site safety procedures, policies, and standard operating procedures.
• Follow experimental protocols carefully, use appropriate controls, and maintainaccurate sample identities and records, including when handling large numbers of samples.
• Assess the quality of samples and experimental results. Investigate unexpected findings, troubleshoot technical problems, and discuss possible explanations and next steps with the project scientist.
• Work independently to prioritize experiments and meet timelines while collaborating with the project scientist and other members of the research group.
• Use spreadsheets, electronic laboratory notebooks, and presentation software to document experiments, track samples, organize data, and communicate results.
• Communicate clearly and respectfully with colleagues, take direction and feedback, and share methods or provide hands-on training when appropriate.
• Ability to work on-site at South San Francisco.

Connaissances

Mammalian cell culture

RNA isolation

RT-qPCR

NGS

RNA quality control

Laboratory techniques

Troubleshooting

Formation

Bachelor’s degree in molecular biology, cell biology, biochemistry, genetics, or related field

Outils

RNA sequencing library prep

Immunoprecipitation

Western blotting

PCR

Transfection

Description du poste

Planet Pharma is seeking a Research Associate to study RNA post-transcriptional processing and regulation in human cells. You will perform experiments, maintain accurate records, and collaborate with the project scientist to interpret results and determine next steps.

The ideal candidate is curious, resourceful, and willing to ask questions, investigate unexpected results, and see experiments through to completion. On-site work in South San Francisco is required.