Research Technologist - 117841

The Johns Hopkins University; Baltimore (MD); On-site; USD 45,000 - 70,000; Full time

Job summary

An established industry player is seeking a Research Technologist to join their dynamic team in the Genetic Medicine department. This role involves handling and analyzing large DNA samples, executing molecular genetics tests, and maintaining laboratory records. The ideal candidate will possess a strong foundation in molecular biology and genetics, along with excellent organizational and communication skills. You'll have the opportunity to work with cutting-edge technology and contribute to innovative research projects that can make a significant impact in the field. If you're passionate about advancing scientific knowledge and thrive in a collaborative environment, this position is perfect for you.

Qualifications

• Basic understanding of molecular biology and genetics required.
• Ability to operate scientific laboratory equipment safely.

Responsibilities

• Transfer DNA samples into 96-well plate format and prepare Deepwell DNA stock plates.
• Perform quality control checks of data using various software.

Skills

Molecular Biology

Genetics

Laboratory Equipment Operation

Data Management

Communication Skills

Education

Bachelor's Degree in Biology

Bachelor's Degree in Chemistry

Tools

Illumina Systems

Genome Studio

Spotfire

Oracle Database

Thermal Cyclers

Job description

Join to apply for the Research Technologist - 117841 role at The Johns Hopkins University

Requisition ID: 117841

Research Technologist

The Johns Hopkins University department of Genetic Medicine is seeking a Research Technologist who will be responsible for sample handling, planning, and executing molecular genetics tests (e.g., genotyping or sequencing) on large numbers of DNA samples.

Specific Duties & Responsibilities

1. Transfer DNA samples into 96-well plate format.
2. Receive and prepare Deepwell DNA stock plates and create working DNA plates.
3. Determine concentration and volume of DNA samples using various instruments.
4. Dilute samples to working concentrations.
5. Maintain and monitor inventory of all in-house DNA samples and reagent supplies.
6. Create master mixes; set up polymerase chain reactions; troubleshoot such reactions.
7. Operate and troubleshoot robotic liquid handlers.
8. Prepare and complete SNP genotyping assays including, but not limited to, Illumina assays and workflows.
9. Prepare samples for targeted DNA capture for sequencing experiments, including library preparation and capture techniques.
10. Prepare and complete Next-Gen sequencing assays, including Illumina NovaSeq workflows.
11. Perform quality control checks of data using various software, including Genome Studio, Spotfire, and other in-house QC metrics reports.
12. Maintain accurate laboratory records (electronic and written).
13. Collect, organize, interpret, and submit experimental data to databases and supervisors.
14. Perform general lab maintenance duties.
15. Perform validation tests of lab reagents.
16. Participate in committees to improve processes.
17. Communicate effectively with supervisor and team members.
18. Perform miscellaneous related duties as assigned.

Special Knowledge, Skills, & Abilities

• Basic understanding of molecular biology and genetics.
• Ability to safely operate scientific laboratory equipment.
• Use of specialized equipment and software, including centrifuges, Illumina systems, Covaris, BioAnalyzer, Nanodrop, thermal cyclers, robotic and manual liquid handlers, Macintosh, PC, UNIX servers, Oracle database.
• Strong organizational skills for time and data management.
• Excellent spoken communication skills.
• Flexibility to work hours starting as early as 6:00 a.m. and/or ending as late as 6:00 p.m.

Minimum Qualifications

• Bachelor's Degree in Biology, Chemistry, or related field.
• Some related post-undergraduate work experience preferred.
• Additional education may substitute for experience, and related experience may substitute for education, as permitted by JHU's equivalency formula.